槲皮素对大鼠中枢剥夺黄体生成素合成270d后肝脏形态和生化变化的影响

M. Rud, V. Shepitko, Y. Stetsuk, O. Akimov
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引用次数: 0

摘要

肝脏炎症过程的发展,包括在乙型肝炎和丙型肝炎病毒的影响下,由免疫系统的细胞控制,即正弦内皮细胞、伊藤细胞和库普弗细胞。巨噬细胞在建立防线方面发挥着关键作用。巨噬细胞的特定群体如何导致肝脏疾病和再生一直是一个争论不休的问题。因此,识别这些人类巨噬细胞群体的特征对于研究它们在肝脏病理发展中的作用具有不可否认的价值。黄酮类槲皮素具有抗氧化和膜稳定作用,具有毛细管稳定特性。我们研究的目的是确定由于在第270天引入醋酸曲普瑞林而抑制雄性大鼠中枢睾酮合成所引起的免疫活性肝细胞的定性和定量变化,以及在先前给予醋酸曲普瑞林溶液的背景下槲皮素对形态学和肝脏抗原呈递细胞计数的潜在影响。实验在30只成年雄性大鼠身上进行。将大鼠分为3组:对照组(10)、实验组I(10)和实验组II(10)。实验组I的动物皮下注射醋酸曲普瑞林,剂量为0.3mg活性物质/kg体重。在实验组II中,动物每周3次接受相同剂量的醋酸曲普瑞林和槲皮素100mg/kg体重,而对照组则给予生理盐水。我们在10%的肝组织匀浆中进行了生化研究。测定了超氧化物阴离子自由基(SAR)的主要产生量和超氧化物歧化酶活性。过氧亚硝酸盐和超氧阴离子自由基是强大的氧化剂,可破坏生物聚合物(DNA、蛋白质和生物膜),并导致氧化亚硝化应激的发展。
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THE EFFECT OF QUERCETIN ON MORPHOLOGICAL AND BIOCHEMICAL CHANGES IN RAT LIVER UNDER 270TH DAY CENTRAL DEPRIVATION OF LUTEINIZING HORMONE SYNTHESIS
The development of the inflammatory process in the liver, including under the influence of hepatitis B and C viruses, is controlled by cells of the immune system, namely, sinusoidal endothelial cells, Ito cells and Kupffer cells. Macrophages play one of the key roles in creating the line of defense. The way in which specific populations of macrophages contribute to liver disease and regeneration is a matter of constant debate. Therefore, identifying the characteristics of these populations of human macrophages is of undeniable value in studying their role in the development of liver pathology. The flavonoid quercetin has capillary-stabilizing properties due to its antioxidant and membrane-stabilizing action. The aim of our study was to determine the changes in immunocompetent liver cells, both qualitative and quantitative, caused by inhibition of central testosterone synthesis in male rats due to the introduction of triptorelin acetate on the 270th day, and the potential effect of quercetin on morphology and liver antigen-presenting cells count against the background of previous administration of triptorelin acetate solution. The experiments were performed on 30 adult male white rats. Rats were divided into 3 groups: control (10), experimental I (10), and experimental II (10). Animals from experimental group I were injected triptorelin acetate subcutaneously at a dose of 0.3 mg of active substance per kg of body weight. In experimental group II, animals received triptorelin acetate in the same dosage and quercetin 100 mg per kg body weight 3 times a week, whereas the control group was administered saline. We conducted biochemical studies in 10% liver tissue homogenate. The main production of superoxide anionic radical (SAR) and superoxide dismutase activity were determined. Peroxynitrite and superoxide anion radical are powerful oxidants that can damage biological polymers (DNA, proteins and biological membranes) and lead to the development of oxidative-nitrosative stress.
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