上皮性牙源性病变中SHH、SMO和gli1蛋白的免疫组织化学分析

Q2 Medicine Brazilian dental journal Pub Date : 2022-10-01 DOI:10.1590/0103-6440202204972
K. S. Rodrigues, H. Santos, E. Morais, R. Freitas
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引用次数: 2

摘要

本研究分析了良性上皮性牙源性病变(牙源性角化囊肿- OKC、成釉细胞瘤- AB和腺瘤样牙源性肿瘤- AOT)中涉及sonic hedgehog信号通路的蛋白(SHH、SMO和gli1)的表达,以确定这些蛋白在这些病变发病机制中的作用。样本包括20个okc, 20个ABs和10个aot。采用Kruskal-Wallis、Mann-Whitney U和Spearman (r)检验进行统计学分析,显著性水平为5% (p < 0.05)。与AOT (p = 0.022)和OKC (p = 0.02)相比,AB中SHH的膜/细胞质表达显著升高。在所研究的病变中,SMO的膜/细胞质表达没有差异。关于gli1,与AOT相比,AB和OKC在核水平上观察到显著差异(p < 0.0001)。此外,AB细胞质与细胞核gli1呈显著正相关(r = 0.482;p = 0.031)和OKC (r = 0.865;p < 0.0001), AOT中膜/细胞质SMO与细胞质gli1之间存在差异(r = 0.667;p = 0.035)和OKC (r = 0.535;P = 0.015)。本研究结果证实了sonic hedgehog信号通路参与了所研究病变的发病机制。抗体中SHH的过表达以及抗体和OKCs中gli1的核表达表明,与AOT相比,这些蛋白有助于这两种病变更具攻击性的行为。
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Immunohistochemical analysis of SHH, SMO and GLI-1 proteins in epithelial odontogenic lesions
Abstract The present study analyzed the expression of proteins involved in the sonic hedgehog signaling pathway (SHH, SMO, and GLI-1) in benign epithelial odontogenic lesions (odontogenic keratocyst - OKC, ameloblastoma - AB, and adenomatoid odontogenic tumor - AOT) in order to identify the role of these proteins in the pathogenesis of these lesions. The sample consisted of 20 OKCs, 20 ABs, and 10 AOTs. The Kruskal-Wallis, Mann-Whitney U, and Spearman’s (r) tests were used for statistical analysis, with the level of significance set at 5% (p < 0.05). The membrane/cytoplasmic expression of SHH was significantly higher in AB compared to AOT (p = 0.022) and OKC (p = 0.02). No differences were found in the membrane/cytoplasmic expression of SMO between the lesions studied. Regarding GLI-1, significant differences were observed at the nuclear level for AB and OKC compared to AOT (p < 0.0001). In addition, significant positive correlations were found between cytoplasmic and nuclear GLI-1 in AB (r = 0.482; p = 0.031) and OKC (r = 0.865; p < 0.0001), and between membrane/cytoplasmic SMO and cytoplasmic GLI-1 in AOT (r = 0.667; p = 0.035) and OKC (r = 0.535; p = 0.015). The results of this study confirm the participation of the sonic hedgehog signaling pathway in the pathogenesis of the lesions studied. Overexpression of SHH in ABs and nuclear expression of GLI-1 in ABs and OKCs indicate that these proteins contribute to the more aggressive behavior of these two lesions when compared to AOT.
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来源期刊
Brazilian dental journal
Brazilian dental journal Dentistry-Dentistry (all)
CiteScore
2.20
自引率
0.00%
发文量
69
审稿时长
12 weeks
期刊介绍: Brazilian Dental Journal, publishes Full-Length Papers, Short Communications and Case Reports, dealing with dentistry or related disciplines and edited six times a year.
期刊最新文献
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