辣椒叶片中的生物蛋白酶抑制剂:作为抗炭疽菌分子的潜在用途

JSFA reports Pub Date : 2023-04-19 DOI:10.1002/jsf2.116
Lídia S. Pereira, Gabriel B. Taveira, Thaynã A. M. Souza, André O. Carvalho, Virginia S. Carvalho, Rosana Rodrigues, Katia Valevski S. Fernandes, Erica O. Mello, Valdirene M. Gomes
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引用次数: 0

摘要

研究背景辣椒UENF1381主要在其种子和果实中发现了多种抗菌分子,对黄单胞菌属细菌和炭疽菌属真菌等多种植物病原微生物具有抗性。然而,对其他植物器官的防御机制知之甚少。本研究旨在通过对玉米主要病原菌炭疽病菌(Colletotrichum scovillei)的抗菌肽的鉴定和鉴定,研究玉米叶片和根系对炭疽病菌(Colletotrichum scovillei)的抗性诱导。结果根据对感染反应的差异蛋白表达结果,选择了LC48(对照叶片模拟物接种48 h)和RC48(对照根模拟物接种48 h)提取物。HPLC系统分析结果显示,从LC48提取物中分离得到23个组分(L1 ~ L23),从RC48提取物中分离得到27个组分(R1 ~ R27)。结果表明,10 μg mL−1的L1组分能够抑制88.4%的真菌生长,导致细胞膜通透性、内源活性氧诱导和线粒体活性降低。L1片段中的肽被命名为CaLPI,与蛋白酶抑制剂的不同序列具有相似性。结论本研究揭示了C. annuum UENF1381叶和根中抗菌蛋白和肽的多样性。有了这些结果,我们希望有助于利用肽作为潜在的分子在微生物控制。
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Biotive protease inhibitor from leaves of Capsicum annuum L.: Potential use as an antifungal molecule against Colletotrichum scovillei

Background

A diverse set of antimicrobial molecules have been found mainly in the seeds and fruits of Capsicum annuum UENF1381, accession resistant to different phytopathogenic microorganisms, like bacteria of the genus Xanthomonas and fungi of the genus Colletotrichum. However, there is little information about the defense mechanisms in other plant organs. The work aimed to study the induction of resistance in leaves and roots of C. annuum through the identification and characterization of antimicrobial peptides against Colletotrichum scovillei, one of the main pathogens of the crop.

Results

Based on the results of differential protein expression in response to infection, the extracts, LC48 (Control Leaf mock inoculated 48 h), and RC48 (Control Root mock inoculated 48 h) were selected. Analysis on the HPLC system resulted in 23 fractions (L1 to L23) obtained from the LC48 extract and 27 fractions from the RC48 extract (R1 to R27). Our results show that 10 μg mL−1 of the L1 fraction is able to inhibit 88.4% of fungal growth, causing cell membrane permeabilization, endogenous reactive oxygen species induction and mitochondrial activity reduction. The peptide in the L1 fraction, named CaLPI, showed similarities with different sequences of proteinase inhibitors.

Conclusion

The study provides information on the diversity of antimicrobial proteins and peptides present in the leaves and roots of C. annuum UENF1381. With these results, we hope to contribute to the use of peptides as potential molecules in microbial control.

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