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Cover: In Bassiouni et al. (http://doi.org/10.1002/cpmo.84), the image shows frozen sections of the C57BL/6J mouse cochlea at p3. To demonstrate the “mouse-on-mouse” background, we omitted incubation with the primary antibody but incubated the sections only with a secondary antibody (goat anti-mouse Alexa Fluor 488). Some slides were blocked with 5% normal goat serum for 30 min. Other slides underwent the same serum blocking step, followed by a second blocking step with an anti-mouse-IgG blocking reagent for 1 hr at RT. (A) Using sole blocking with serum resulted in a strong background fluorescence signal, most prominently in the spiral limbus, and a lateral cochlear wall. A moderate signal was seen in the basilar membrane, intercellular borders, spiral lamina, and in the capillaries of the stria vascularis. (B) Using double-blocking resulted in no background fluorescence signal under identical exposure time and microscopy conditions. Scale: 20 µm.