瓜沙对大鼠刮皮组织形态学及降钙素基因相关肽和P物质表达的影响

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-08-01 DOI:10.1016/S0254-6272(18)30888-4
Wang Yingying , Xu Dongsheng , Bai Wanzhu , Yang Jinsheng
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引用次数: 2

摘要

目的探讨刮痧对大鼠刮伤皮肤组织形态学及降钙素基因相关肽(CGRP)和P物质(SP)表达的影响。方法50只大鼠作为实验对象,根据不同观察时间点随机分为5组。为了暴露脊柱两侧的皮肤,刮去背刚毛。瓜沙组大鼠在脊柱一侧相当于人体膀胱经络的部位采用均匀补泻法,自上至下刮拭,直至出疹子。灌注后取有麻疹疹的皮肤组织。取A组大鼠相应部位作为对照。将组织切片,对SP、CGRP等抗体进行免疫荧光组织化学染色和HE染色。结果瓜沙治疗后,大鼠背部局部刮擦皮肤外观、毛囊及血管与对照组相比均有显著差异;在不同的时间点,差异减小。不同时间点大鼠背部局部刮擦皮肤CGRP和SP的表达差异无统计学意义。结论瓜沙对局部皮肤内神经纤维形态及毛囊、血管等组织形态无明显影响。然而,瓜沙之后,他们在五天内基本恢复了正常水平。
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Effects of Guasha on histomorphology of scraped skins and on expression of calcitonin gene-related peptide and substance P in rats

OBJECTIVE

To reveal the effects of Guasha (scraping therapy) on the histomorphology of scraped skins and on the expressions of calcitonin gene-related peptide (CGRP) and substance P (SP).

METHODS

50 rats, as experimental subjects, were randomly divided into 5 groups according to different observation time points. Dorsal setae were shaved for the exposure of skin on both sides of the spine. Even reinforcing and reducing method was applied to the rats in Guasha groups on the site equivalent to bladder meridian of human body on one side of the spine, and the skin was scraped from top to bottom until rash of measles occurred. The skin tissues with rash of measles were taken down after perfusion. The corresponding sites of rats in group A were also taken down as control. The tissues were made to sections and used for immunofluorescence histochemical staining and HE staining of antibodies such as SP and CGRP.

RESULTS

After Guasha, there were significant differences in appearance, hair follicle and blood vessel on local skin scraped on the back of rats when compared with control; In different time points, the differences reduced. There was no significant difference in expression of CGRP and SP when compared local skin scraped on the back of rats in different time points.

CONCLUSION

Guasha didn't significantly change the morphology of nerve fibers inside local skin, and the histomorphology of hair follicle, blood vessel and etc. However, after Guasha they basically returned to normal level within five days.

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来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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