Su Weiyang , Feng Chienwei , Tseng Chungchih , Hung Hanchun , Chen Zhicheng , Lee Hsinpai , Jean Yenhsuan , Wu Shingyi Sean , Wen Zhihong
{"title":"归甲胶对大鼠和斑马鱼骨再生的治疗作用","authors":"Su Weiyang , Feng Chienwei , Tseng Chungchih , Hung Hanchun , Chen Zhicheng , Lee Hsinpai , Jean Yenhsuan , Wu Shingyi Sean , Wen Zhihong","doi":"10.1016/j.jtcm.2018.04.006","DOIUrl":null,"url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effect of Guijiajiao (<em>Colla Carapacis et Plastri,</em> CCP), the glue of tortoise shell, on bone regeneration in zebrafish and male Wistar rats.</p></div><div><h3>METHODS</h3><p>In this study, we applied <em>in vitro</em> and <em>in vivo</em> models, tissue section analysis, and quantitative polymerase chain reaction to assess the effects of CCP on bone repair. MG-63 cells were used in alkaline phosphatase (ALP) activity and mineralization assays. Zebrafish and male Wistar rats were used to evaluate the effects of CCP on bone repair <em>in vivo.</em></p></div><div><h3>RESULTS</h3><p>A simple preparation of CCP promoted osteogenesis <em>in vivo</em> and <em>in vitro,</em> and promoted MG-63 cell proliferation as well as ALP activity and mineralization. In addition, CCP activated Akt and extracellular signaling-regulated kinase pathways and significantly increased the expression of ossification-related genes and proteins such as runt-related transcription factor-2, osteocalcin, and osteopontin. The <em>in vivo</em> results revealed promotion of osteogenesis by immersing zebrafish in CCP for 72 h. An oral dose of 1.25 g/kg CCP significantly improved skull defects in rats, which was accompanied by an increase in serum ALP levels.</p></div><div><h3>CONCLUSION</h3><p>One of the ingredients of Guilu Erxianjiao (tortoise shell and deer horn formula) provides a practical alternative therapy for bone regeneration.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.04.006","citationCount":"5","resultStr":"{\"title\":\"Therapeutic effect of Guijiajiao (Colla Carapacis et Plastri) on bone regeneration in rats and zebrafish\",\"authors\":\"Su Weiyang , Feng Chienwei , Tseng Chungchih , Hung Hanchun , Chen Zhicheng , Lee Hsinpai , Jean Yenhsuan , Wu Shingyi Sean , Wen Zhihong\",\"doi\":\"10.1016/j.jtcm.2018.04.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>OBJECTIVE</h3><p>To investigate the effect of Guijiajiao (<em>Colla Carapacis et Plastri,</em> CCP), the glue of tortoise shell, on bone regeneration in zebrafish and male Wistar rats.</p></div><div><h3>METHODS</h3><p>In this study, we applied <em>in vitro</em> and <em>in vivo</em> models, tissue section analysis, and quantitative polymerase chain reaction to assess the effects of CCP on bone repair. MG-63 cells were used in alkaline phosphatase (ALP) activity and mineralization assays. Zebrafish and male Wistar rats were used to evaluate the effects of CCP on bone repair <em>in vivo.</em></p></div><div><h3>RESULTS</h3><p>A simple preparation of CCP promoted osteogenesis <em>in vivo</em> and <em>in vitro,</em> and promoted MG-63 cell proliferation as well as ALP activity and mineralization. In addition, CCP activated Akt and extracellular signaling-regulated kinase pathways and significantly increased the expression of ossification-related genes and proteins such as runt-related transcription factor-2, osteocalcin, and osteopontin. The <em>in vivo</em> results revealed promotion of osteogenesis by immersing zebrafish in CCP for 72 h. An oral dose of 1.25 g/kg CCP significantly improved skull defects in rats, which was accompanied by an increase in serum ALP levels.</p></div><div><h3>CONCLUSION</h3><p>One of the ingredients of Guilu Erxianjiao (tortoise shell and deer horn formula) provides a practical alternative therapy for bone regeneration.</p></div>\",\"PeriodicalId\":17513,\"journal\":{\"name\":\"Journal of Traditional Chinese Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2018-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.04.006\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Traditional Chinese Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0254627218303029\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Traditional Chinese Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254627218303029","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
Therapeutic effect of Guijiajiao (Colla Carapacis et Plastri) on bone regeneration in rats and zebrafish
OBJECTIVE
To investigate the effect of Guijiajiao (Colla Carapacis et Plastri, CCP), the glue of tortoise shell, on bone regeneration in zebrafish and male Wistar rats.
METHODS
In this study, we applied in vitro and in vivo models, tissue section analysis, and quantitative polymerase chain reaction to assess the effects of CCP on bone repair. MG-63 cells were used in alkaline phosphatase (ALP) activity and mineralization assays. Zebrafish and male Wistar rats were used to evaluate the effects of CCP on bone repair in vivo.
RESULTS
A simple preparation of CCP promoted osteogenesis in vivo and in vitro, and promoted MG-63 cell proliferation as well as ALP activity and mineralization. In addition, CCP activated Akt and extracellular signaling-regulated kinase pathways and significantly increased the expression of ossification-related genes and proteins such as runt-related transcription factor-2, osteocalcin, and osteopontin. The in vivo results revealed promotion of osteogenesis by immersing zebrafish in CCP for 72 h. An oral dose of 1.25 g/kg CCP significantly improved skull defects in rats, which was accompanied by an increase in serum ALP levels.
CONCLUSION
One of the ingredients of Guilu Erxianjiao (tortoise shell and deer horn formula) provides a practical alternative therapy for bone regeneration.
期刊介绍：
Journal of Traditional Chinese Medicine（JTCM） is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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