西桑甲醇提取物对Wistar大鼠抗增殖能力的评估

Q4 Agricultural and Biological Sciences Journal of Phytology Pub Date : 2021-12-23 DOI:10.25081/jp.2021.v13.7247
Dayo Omotoso, U. Okwuonu, O. Lawal, Oluwasegun Davis Olatomide
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引用次数: 0

摘要

西桑是一种药用植物,具有广泛的治疗用途。在这项研究中,目的是利用细胞增殖和肿瘤抑制标志物进一步评估其抗增殖潜力。以甲醇为溶剂提取剂,研究了水芹叶的提取工艺。选取雄性白化Wistar大鼠20只,随机分为4组1 ~ 4 (n=5),分别以0、100、300、500 mg/kg的剂量给药,连续30 d。治疗后,对肝组织进行组织学和免疫组织化学检查,检测细胞增殖(Ki-67)和肿瘤抑制蛋白(p53)。采用image-J软件对蛋白的免疫表达进行定量分析,数据采用SPSS version 20进行分析,数值比较采用t检验和单因素方差分析。组织学结果显示,治疗组2 ~ 4与未治疗组1相比,肝脏组织形态无明显变化。免疫组化结果显示,与未治疗组1相比,治疗组2 ~ 4肝组织中Ki-67蛋白表达显著下调(p< 0.05), p53蛋白表达显著上调(p< 0.05)。这些细胞增殖和肿瘤抑制蛋白的反向表达模式暴露于石竹的甲醇提取物后可能提示植物提取物的抗增殖潜力。
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Assessment of the antiproliferative potential of Cissampelos owariensis (P. Beauv) methanolic extract in Wistar rats
Cissampelos owariensis is a medicinal plant with a wide range of therapeutic uses.  In this study, the objective was to further assess its antiproliferative potential using cell proliferation and tumor suppressor markers. Solvent extraction of C. owariensis leaves was done using methanol. Twenty (20) male albino Wistar rats were randomly divided into four groups 1–4 (n=5) and respectively administered with methanolic extracts of C. owariensis at 0, 100, 300 and 500 mg/kg for 30 days. After treatment, the hepatic tissues were processed and examined histologically and immunohistochemically for cell proliferation (Ki-67) and tumor suppressor (p53) proteins.  Immunoexpression of the proteins was quantified using image-J software, the data analyzed with SPSS version 20 and values compared using t-test and one-way analysis of variance. The histological results showed no significant variation in hepatic histomorphology of treated Groups 2–4 relative to non-treated Group 1. However, the immunohistochemical results showed significant (p< 0.05) down-regulation in Ki-67 protein expression and a concomitant significant (p< 0.05) up-regulation in p53 protein expression in hepatic tissues of treated Groups 2–4 relative to non-treated Group 1.These inverse expression patterns of cell proliferation and tumor suppressor proteins following exposure to methanolic extracts of C. owariensis may suggest the antiproliferative potential of the plant extracts.
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来源期刊
Journal of Phytology
Journal of Phytology Agricultural and Biological Sciences-Plant Science
CiteScore
1.40
自引率
0.00%
发文量
17
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