Antioxidant Activity and Polyphenols Content of Artemisia Herba-Alba Extract and their Cytotoxicity against Human Lung Cancer Cells NCI-N417

ABSTRACT Phytochemicals in the ethanol extract of Artemisia herba-alba were examined and evaluated for the antioxidant and cytotoxicity potential. The extraction was performed using an accelerated solvent extraction (ASE300), analyzed by GC-MS and the total phenolics was estimated by Folin–Ciocalteau method. The antioxidant activity of the extract was evaluated using three methods and its cytotoxicity was evaluated in-vitro on NCI-N417 tumor cell line using MTT test and flow cytometry. Twenty-one compounds were identified in the A. herba-alba extract, which was rich in phenolic compounds (248.56 ± 11.05 μg GAE mg−1 extract) and had antioxidant activity characterized by a high DPPH-radical-scavenging activity (IC50 = 69.01 ± 0.03 μg mL−1), inhibitory effect of β-carotene oxidation (95.75%±0.013), and reducing power (DO700nm = 0.751 ± 0.001). Ethanol extract from A. herba-alba demonstrated cytotoxic and antiproliferative activity.