Haplotype Diversity and Molecular Phylogeny of Wild Crambe L. (Brassicaceae) Taxa of Turkey

: Revealing genetic diversity is both essential for plant systematics and also provides important information for agricultural sciences. Crambe hispanica var. abyssinica is an oil seed crop. Wild plants related to crops (Crop Wild Relatives) are important resources for the genetic improvement of cultivated species. In order to reveal the genetic diversity of wild Crambe taxa of Turkey, we used ITS and trn L-F markers to create the haplotype networks and phylogeny reconstruction. Thirty-nine accessions belonging to 8 Crambe taxa were used as the material of our study: Crambe orientalis var . orientalis with 18 accessions, Crambe orientalis var . sulphurea with 3 accessions, Crambe orientalis var . dasycarpa with 1 accession, Crambe alutacea with 1 accession, Crambe grandiflora with 1 accession, Crambe tataria var . tataria with 8 accessions, Crambe tataria var. aspera with 2 accessions, and Crambe maritima with 5 accessions. The phylogeny inference of ITS and trn L-F data revealed two major lineages: one consisted of Crambe maritima and Crambe tataria accessions, while the other consisted of Crambe orientalis subsp . orientalis , Crambe orientalis subsp . sulphurea , Crambe orientalis var . dasycarpa , Crambe grandiflora , and Crambe alutacea. In the haplotype networks based trn L-F region, all Crambe maritima accessions and most of the Crambe. orientalis accessions shared one haplotype. However, Crambe maritima , Crambe orientalis , and Crambe tataria shared no common haplotype in the networks based on ITS region. In both networks, Crambe alutacea shared one haplotype with some Crambe orientalis . Crambe maritima and Crambe orientalis shared the H1 haplotype . Other haplotypes differed from the most common haplotype (H1) by one or two base pairs. Crambe orientalis is the species with the highest haplotype diversity and IT6 haplotype has the highest seed oil content among CWR of Crambe in Turkey.