流式细胞术检测细胞内SLAMF受体相关接头蛋白SAP和EAT-2的优化

European Pharmaceutical Journal Pub Date : 2022-06-18 DOI:10.2478/afpuc-2022-0007

Mykhailo Huniadi, S. Salanci, Jan Sykora, S. Kurhajec, M. Šarišský

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引用次数: 0

摘要

SAP和EAT-2是连接SLAMF受体到下游细胞内信号通路的细胞内衔接蛋白。它们的表达和功能在不同类型的造血细胞中有所不同。SAP和EAT-2在血液恶性肿瘤中的作用尚不清楚。在这里，我们试图优化一种流式细胞术检测细胞内SAP和EAT-2的方法。我们发现使用PerFix EXPOSE试剂盒进行细胞固定和渗透可确保在染色强度和信噪比方面获得最佳结果。

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Optimisation of flow cytometric detection of intracellular SLAMF receptor-associated adaptor proteins SAP and EAT-2

Abstract SAP and EAT-2 are intracellular adaptor proteins that link SLAMF receptors to downstream intracellular signalling pathways. Their expression and functions vary among different types of hematopoietic cells. The roles of SAP and EAT-2 in haematological malignancies are poorly understood. Here, we attempted to optimise a method for the detection of intracellular SAP and EAT-2 by flow cytometry. We found that using PerFix EXPOSE kit for cell fixation and permeabilization ensures optimal results in terms of staining intensity and signal-to-noise ratio.

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来源期刊

European Pharmaceutical Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： European Pharmaceutical Journal publishes only original articles not previously published and articles that are not being considered or have not been submitted for publication elsewhere. If parts of the results have been published as conference abstract or elsewhere, it should be stated in references. The ethical standards of the Helsinki-Tokio Declaration should be kept. This should be mentioned in the Methods of manuscript. Reviews are published only on request. Authors, whose submitted research work was performed with the support of a company, should indicate this in Conflict of Interest.