Hemiao Zhao, Qingluan Lin, Qi Zhang, Jing Chen, Z. Tu, Ruiqin Yang, Lan Hu, Chong Wang
{"title":"不同染色载体对血迹mrna谱的影响","authors":"Hemiao Zhao, Qingluan Lin, Qi Zhang, Jing Chen, Z. Tu, Ruiqin Yang, Lan Hu, Chong Wang","doi":"10.4103/jfsm.jfsm_36_21","DOIUrl":null,"url":null,"abstract":"Unlike DNA profiling, mRNA profiling is greatly affected by external factors. To analyze the influence of different stain carriers on the detectability of mRNA markers from bloodstains, this study examined 10 carriers, including a knife, cotton swab, paper, plastic, leather, cement, chopsticks, clothes, ceramic block, and wall. After detecting five specific mRNA markers (HBA, HBB, ALAS2, GYPA, and SPTB) and the housekeeping gene B2M in peripheral blood samples, no statistically significant differences in the effects of the carriers were found. The results suggest that when performing mRNA testing on bloodstains, the effect of the stain carrier has little influence.","PeriodicalId":36434,"journal":{"name":"Journal of Forensic Science and Medicine","volume":"8 1","pages":"76 - 79"},"PeriodicalIF":0.0000,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The impact of different stain carriers on the mrna profiling from bloodstains\",\"authors\":\"Hemiao Zhao, Qingluan Lin, Qi Zhang, Jing Chen, Z. Tu, Ruiqin Yang, Lan Hu, Chong Wang\",\"doi\":\"10.4103/jfsm.jfsm_36_21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Unlike DNA profiling, mRNA profiling is greatly affected by external factors. To analyze the influence of different stain carriers on the detectability of mRNA markers from bloodstains, this study examined 10 carriers, including a knife, cotton swab, paper, plastic, leather, cement, chopsticks, clothes, ceramic block, and wall. After detecting five specific mRNA markers (HBA, HBB, ALAS2, GYPA, and SPTB) and the housekeeping gene B2M in peripheral blood samples, no statistically significant differences in the effects of the carriers were found. The results suggest that when performing mRNA testing on bloodstains, the effect of the stain carrier has little influence.\",\"PeriodicalId\":36434,\"journal\":{\"name\":\"Journal of Forensic Science and Medicine\",\"volume\":\"8 1\",\"pages\":\"76 - 79\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Forensic Science and Medicine\",\"FirstCategoryId\":\"90\",\"ListUrlMain\":\"https://doi.org/10.4103/jfsm.jfsm_36_21\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Social Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Forensic Science and Medicine","FirstCategoryId":"90","ListUrlMain":"https://doi.org/10.4103/jfsm.jfsm_36_21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Social Sciences","Score":null,"Total":0}
The impact of different stain carriers on the mrna profiling from bloodstains
Unlike DNA profiling, mRNA profiling is greatly affected by external factors. To analyze the influence of different stain carriers on the detectability of mRNA markers from bloodstains, this study examined 10 carriers, including a knife, cotton swab, paper, plastic, leather, cement, chopsticks, clothes, ceramic block, and wall. After detecting five specific mRNA markers (HBA, HBB, ALAS2, GYPA, and SPTB) and the housekeeping gene B2M in peripheral blood samples, no statistically significant differences in the effects of the carriers were found. The results suggest that when performing mRNA testing on bloodstains, the effect of the stain carrier has little influence.
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