Antibacterial efficacy of the combination of triphala and chlorhexidine mouthwashes on salivary Streptococcus mutans of orthodontic patients: A randomized clinical trial

Aim: The aim of this study was to compare and evaluate the antibacterial efficacy of triphala mouthwash and its combination with 0.2% chlorhexidine against salivary Streptococcus mutans of orthodontic patients. Materials and Methods: A total of 54 patients under fixed orthodontic therapy were included in this randomized clinical trial and divided into three groups of 18 each. The groups were requested to use one of three mouthwashes two times a day for 10 days: chlorhexidine 0.2%, triphala 25%, and the combination of chlorhexidine and triphala (1:1). Saliva samples were collected at baseline (day 0) and after 10 days. Ultimately, the samples were diluted and cultured. After incubation, the formed colonies were counted and the CFU/mL was calculated. The results were analyzed statistically with one-way analysis of variance and t test. Results: All mouthwashes decreased the bacterial count from baseline to the end of 10 days (P < 0.05). Although triphala had the least antimicrobial effect, no significant difference was observed between the chlorhexidine and the combined mouthwash group (P = 0.251). Conclusion: It was concluded that the combination of chlorhexidine and triphala reduces the side effects of chlorhexidine while presenting an acceptable antibacterial efficacy.