O. El-Guourrami, Soufiane Drioua, Mouna Ameggouz, N. Salhi, K. Sayah, G. Zengin, Ahmed Zahidi, A. Doukkali, H. Benzeid
{"title":"麻仁提取物的抗氧化、镇痛活性及植物化学分析","authors":"O. El-Guourrami, Soufiane Drioua, Mouna Ameggouz, N. Salhi, K. Sayah, G. Zengin, Ahmed Zahidi, A. Doukkali, H. Benzeid","doi":"10.21448/ijsm.1139246","DOIUrl":null,"url":null,"abstract":"Ammi majus (L.) is commonly used to cure many diseases in Moroccan folk medicine, especially vitiligo. This research tries to evaluate the phytochemical constituents of two aqueous extracts (E1; Maceration; 48 h) and (E2: Infusion; 1h) and three organic fractions (F1: Cyclohexane), (F2: Ethyl acetate (EtOAc)) and (F3: Ethanolic (EtOH)) of A. majus (L.) seeds, as well as to study the antioxidant and analgesic activity of the species. Phytochemical analysis, antioxidant activity (DPPH, FRAP, ABTS, and TAC tests), and analgesic activity (writhing and tail immersion were induced by Acetic acid tests) were analyzed according to the literature. A quantitative phytochemical study indicate that the E1 had the highest content of total polyphenols (26.95 ± 0.53 mg GAE/g extract) and flavonoids (37.92 ± 0.46 mg QE/g extract), while F3 showed a promising flavonol content (24.26±0.08 mg QE/g extract). Tannins were found to be high in F1 (59.27 ± 0.16 mg CE/g extract) and F2 (57.65 ± 1.18 mg CE/g extract). Antioxidant results reveals that DPPH (IC50 = 179.68 ± 0.47 μg/mL) and FRAP (EC50 = 367.03 ± 0.12 μg/mL) show to E1 a high antioxidant activity. Regarding the analgesic activity of the different studied extracts, it was found that E1 has a high peripheral analgesic effect with 62.32 % and a high central analgesic potential throughout the experimentation at 500 mg/kg. Our studies demonstrated for the first time that A. majus seeds extracts have high antioxidant and analgesic activities through different analysis techniques.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Antioxidant activity, analgesic activity, and phytochemical analysis of Ammi majus (L.) extracts\",\"authors\":\"O. El-Guourrami, Soufiane Drioua, Mouna Ameggouz, N. Salhi, K. Sayah, G. Zengin, Ahmed Zahidi, A. Doukkali, H. Benzeid\",\"doi\":\"10.21448/ijsm.1139246\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Ammi majus (L.) is commonly used to cure many diseases in Moroccan folk medicine, especially vitiligo. This research tries to evaluate the phytochemical constituents of two aqueous extracts (E1; Maceration; 48 h) and (E2: Infusion; 1h) and three organic fractions (F1: Cyclohexane), (F2: Ethyl acetate (EtOAc)) and (F3: Ethanolic (EtOH)) of A. majus (L.) seeds, as well as to study the antioxidant and analgesic activity of the species. Phytochemical analysis, antioxidant activity (DPPH, FRAP, ABTS, and TAC tests), and analgesic activity (writhing and tail immersion were induced by Acetic acid tests) were analyzed according to the literature. A quantitative phytochemical study indicate that the E1 had the highest content of total polyphenols (26.95 ± 0.53 mg GAE/g extract) and flavonoids (37.92 ± 0.46 mg QE/g extract), while F3 showed a promising flavonol content (24.26±0.08 mg QE/g extract). Tannins were found to be high in F1 (59.27 ± 0.16 mg CE/g extract) and F2 (57.65 ± 1.18 mg CE/g extract). Antioxidant results reveals that DPPH (IC50 = 179.68 ± 0.47 μg/mL) and FRAP (EC50 = 367.03 ± 0.12 μg/mL) show to E1 a high antioxidant activity. Regarding the analgesic activity of the different studied extracts, it was found that E1 has a high peripheral analgesic effect with 62.32 % and a high central analgesic potential throughout the experimentation at 500 mg/kg. Our studies demonstrated for the first time that A. majus seeds extracts have high antioxidant and analgesic activities through different analysis techniques.\",\"PeriodicalId\":14437,\"journal\":{\"name\":\"International Journal of Secondary Metabolite\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-03-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Secondary Metabolite\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21448/ijsm.1139246\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Secondary Metabolite","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21448/ijsm.1139246","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Antioxidant activity, analgesic activity, and phytochemical analysis of Ammi majus (L.) extracts
Ammi majus (L.) is commonly used to cure many diseases in Moroccan folk medicine, especially vitiligo. This research tries to evaluate the phytochemical constituents of two aqueous extracts (E1; Maceration; 48 h) and (E2: Infusion; 1h) and three organic fractions (F1: Cyclohexane), (F2: Ethyl acetate (EtOAc)) and (F3: Ethanolic (EtOH)) of A. majus (L.) seeds, as well as to study the antioxidant and analgesic activity of the species. Phytochemical analysis, antioxidant activity (DPPH, FRAP, ABTS, and TAC tests), and analgesic activity (writhing and tail immersion were induced by Acetic acid tests) were analyzed according to the literature. A quantitative phytochemical study indicate that the E1 had the highest content of total polyphenols (26.95 ± 0.53 mg GAE/g extract) and flavonoids (37.92 ± 0.46 mg QE/g extract), while F3 showed a promising flavonol content (24.26±0.08 mg QE/g extract). Tannins were found to be high in F1 (59.27 ± 0.16 mg CE/g extract) and F2 (57.65 ± 1.18 mg CE/g extract). Antioxidant results reveals that DPPH (IC50 = 179.68 ± 0.47 μg/mL) and FRAP (EC50 = 367.03 ± 0.12 μg/mL) show to E1 a high antioxidant activity. Regarding the analgesic activity of the different studied extracts, it was found that E1 has a high peripheral analgesic effect with 62.32 % and a high central analgesic potential throughout the experimentation at 500 mg/kg. Our studies demonstrated for the first time that A. majus seeds extracts have high antioxidant and analgesic activities through different analysis techniques.