体外受精中微流体与游动精子分离方法的比较

Q4 Biochemistry, Genetics and Molecular Biology Medical Journal of Cell Biology Pub Date : 2020-12-31 DOI:10.2478/acb-2020-0022
M. Ješeta, K. Franzová, J. Žáková, P. Ventruba, I. Crha
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引用次数: 1

摘要

精子分离用于ICSI是实现试管婴儿程序的重要步骤。近年来,利用芯片进行精子细胞微流体分离的方法得到了越来越多的应用。与传统方法相比,这种方法通常被认为对精子非常温和,并显著降低了DNA片段精子细胞的浓度。我们研究的目的是从微流控芯片系统选择具有非片段DNA的精子的潜力的角度来验证该系统。我们测试了这种分离方法与上升法的效率。在本研究中,我们评估了10名患者在分离方法前后精子DNA的完整性。同时采用向上游动法和微流控芯片法对每位患者的唾液进行分离。研究表明,这两种方法在减少DNA片段的精子方面非常相似。有趣的是,在所有患者中,微流体分离后DNA片段精子的浓度都低于向上游动法后。然而,这些差异在统计上并不显著,平均只有2.1%,就实际使用而言,这是可以忽略不计的。运行标题:微流控芯片和DNA片段
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Comparison of microfluidic and swim-up sperm separation methods for IVF
Abstract Sperm separation for ICSI is an essential step in realization of the IVF procedures. The method of microfluidic separation of sperm cells using chips has been applied more and more frequently in recent years. This method is often presented as extremely gentle to spermatozoa and decreasing significantly concentration of sperm cells with fragmented DNA when compared to conventional methods. The aim of our study was to verify a microfluidic chip system from the perspective of its potential to select spermatozoa with non-fragmented DNA. We tested the efficiency of this separation method against the swim-up method. In this study we evaluated sperm DNA integrity before and after the separation methods in ten patients. Ejaculate of each patient was separated by both the swim up method and the microfluidic chip method at the same time. It was shown that both the methods are very similar in reduction of spermatozoa with fragmented DNA. Interestingly, the concentration of spermatozoa with fragmented DNA was lower after the microfluidic separation than after the swim-up method in all the patients. Nevertheless, the differences were not statistically significant with only 2.1% on average, which is negligible in terms of practical use. Running title: Microfluidic chip and DNA fragmentation
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来源期刊
Medical Journal of Cell Biology
Medical Journal of Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology
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发文量
15
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