微波辅助液压蒸馏法提取哥伦比亚姜精油挥发物及抗氧化活性分析

IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY Revista Colombiana de Investigaciones Agroindustriales Pub Date : 2021-06-08 DOI:10.23850/24220582.3465
Tapiero-Cuellar Jose Libardo, Guillermo Salamanca-Grosso, M. Benitez
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引用次数: 0

摘要

姜(Zingiber officinale)是姜科植物,其根茎因其辛辣的香气和风味而被广泛应用于美食中。精油(EO)是天然化合物;它们具有25至70种不同浓度的组分。Α-姜烯和α-Felandrene是体积在20%至70%之间的主要成分。为了提取来自哥伦比亚10个地理位置的AE样品,通过微波辐射水蒸馏(MWHD)获得,并在4°C下储存在琥珀色小瓶中。将50µL EA和1.0µL(正十四烷)内标物溶于二氯甲烷中,最终体积为1.0 mL。EO组分在三重四重质量检测器气相色谱仪(BRUKER 450GC-320)上进行。对于其抗氧化活性(AA),使用Prior的DPPH*和ABTS+方法。提取物显示出在0.053%和0.109%之间的产率;并且使用300g 700瓦和40分钟处理的样品。色谱图谱显示43种成分涉及酮、醇、环醚、醛和23种碳氢化合物,其中α-姜烯占大多数,被认为是该EO的分子标记。AA在所研究地区的评估样本之间存在显著差异。
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Analysis of volatile compounds and antioxidant activity of Colombian ginger (Zingiber officinale) essential oil obtained by hydrohydraulic distillation assisted by microwave radiation
The (Zingiber officinale) is a plant of the zingiberáceas family, its rhizome is widely used in gastronomy for its pungent aroma and flavor. Essential oils (EO) are natural compounds; they have 25 to 70 components with different concentrations. Α-Zingiberene and α-Felandrene are the main components with volumes between 20% and 70%. For the extraction of the AE samples from 10 Colombian geographical locations were used, obtained by microwave radiated hydrodistillation (MWHD) and stored at 4°C in amber vials. 50 µL of EA and 1.0 µL of (n-tetradecane) internal standard were dissolved in dichloromethane to a final volume of 1.0 mL. The EO components were performed on a triple quadruple mass detector gas chromatograph (BRUKER 450GC-320). For its antioxidant activity (AA) the methodology of Prior for DPPH* and ABTS+ was used. The extractions showed a yield between 0.053 and 0.109 percent; and 300 g of sample at 700 watts and 40 minutes of process were used. The chromatographic profile showed 43 components referring to ketones, alcohols, cyclic ethers, aldehydes and 23 hydrocarbons, with α-Zingiberene being the majority, considered as a molecular marker of this EO. The AA presents significant differences between the samples evaluated for the localities studied.
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