农杆菌介导洋葱表皮细胞转化的高效瞬时检测系统

IF 1 4区 生物学 Q3 PLANT SCIENCES Indian Journal of Genetics and Plant Breeding Pub Date : 2020-09-22 DOI:10.31742/IJGPB.80.3.17
Zhang Yumiao, Wang Jun, Wu Tao
{"title":"农杆菌介导洋葱表皮细胞转化的高效瞬时检测系统","authors":"Zhang Yumiao, Wang Jun, Wu Tao","doi":"10.31742/IJGPB.80.3.17","DOIUrl":null,"url":null,"abstract":"In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells\",\"authors\":\"Zhang Yumiao, Wang Jun, Wu Tao\",\"doi\":\"10.31742/IJGPB.80.3.17\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.\",\"PeriodicalId\":13321,\"journal\":{\"name\":\"Indian Journal of Genetics and Plant Breeding\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2020-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Genetics and Plant Breeding\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.31742/IJGPB.80.3.17\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Genetics and Plant Breeding","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.31742/IJGPB.80.3.17","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 1

摘要

本研究对农杆菌感染培养基、感染时间、洗涤剂和细胞密度进行了优化。以高粱为基础的侵染培养基(SbIM),侵染时间为10 ~ 20 min,添加0.01%的Silwet L-77,在600 nm光密度(OD600)的条件下,洋葱表皮细胞支持农杆菌侵染的能力提高了50% ~ 90%。细胞周期蛋白依赖性激酶D-2 (CDKD-2)和细胞色素c型生物发生蛋白(CYCH),蛋白-蛋白相互作用定位。优化后的程序是一种快速有效的检测蛋白质亚细胞定位和蛋白质-蛋白质相互作用的系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells
In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
1.80
自引率
10.00%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.
期刊最新文献
Assessment of agro-morphological traits and yield-based tolerance indices in sesame (Sesamum indicum L.) genotypes under drought stress Genetic characterization and comparative assessment of response of biparental and multi-parental populations to northern corn leaf blight in maize (Zea mays L.) Identification of efficient learning classifiers for discrimination of coding and non-coding RNAs in plant species Molecular marker assisted confirmation of hybrids in coconut (Cocos nucifera L.) Influence of G x E interaction on heterobeltiosis, combining ability and stability of multiple cross derivatives of cotton (Gossypium hirsutum L.) amenable for HDPS
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1