经验证的紫外高效液相色谱法测定人脑脊液中5-羟基吲哚乙酸的死后含量

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Journal of Laboratory Physicians Pub Date : 2023-03-14 DOI:10.1055/s-0043-1774378
Divya Bhushan, Jayanthi Yadav, A. Arora, Dhruv Agrawal, Achala Apte
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引用次数: 0

摘要

抽象目标 本研究的目的是开发一种紫外高效液相色谱(UV-HPLC)方法来测量人脑脊液(CSF)中的5-羟基吲哚乙酸(5-HIAA),作为神经和精神疾病的潜在生物标志物,包括具有自杀行为的抑郁障碍。方法 这项研究使用了来自法医尸检的个人的脑脊液样本。5-HIAA浓度使用UV-HPLC法用三种流动相溶剂测量。然后使用最有效的流动相溶剂来测量CSF样品中的5-HIAA。材料 研究中使用的材料包括从法医尸检的个人身上获得的CSF样本、UV-HPLC设备和流动相溶剂,包括5-羟基吲哚-3-乙酸(Merck/sigma)、乙腈、浓甲酸、浓乙酸、甲醇和磷酸。统计分析 这是使用R Studio(版本4.2.0)完成的。结果 研究发现,使用甲酸(0.05–0.1%):乙腈1:9作为流动相的UV-HPLC方法对测量人CSF中的5-HIAA最有效。该方法具有良好的线性、准确度和精密度。结论 研究表明,所建立的紫外-高效液相色谱法测定人脑脊液中5-HIAA是可靠有效的。测量CSF中的5-HIAA水平可以作为神经和精神疾病的潜在生物标志物,包括具有自杀行为的抑郁症。这种方法在临床和法医实践中诊断自杀病例是很有前途的。需要进一步的研究来确定这些发现的临床意义以及在精神病学中更广泛应用的潜力。本文有助于提供一种实用、经济的检测CSF 5-HIAA的方法。
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Validated Ultraviolet High-Performance Liquid Chromatography Method for Post-mortem 5-Hydroxy-indoleacetic Acid Measurement in Human Cerebrospinal Fluid
Abstract Objective  The objective of this study was to develop an ultraviolet high-performance liquid chromatography (UV-HPLC) method for the measurement of 5-hydroxyindoleacetic acid (5-HIAA) in human cerebrospinal fluid (CSF) as a potential biomarker for neurological and psychiatric illnesses, including depressive disorders with suicidal behavior. Methods  The study utilized CSF samples from individuals brought for medicolegal autopsy. The 5-HIAA concentration was measured using a UV-HPLC method with three mobile-phase solvents. The most effective mobile phase solvent was then used to measure 5-HIAA in the CSF samples. Materials  The materials used in the study included CSF samples obtained from individuals brought for medico-legal autopsy, UV-HPLC equipment, and mobile phase solvents, including 5-hydroxyindole-3-acetic acid (Merck/sigma), acetonitrile, concentrated formic acid, concentrated acetic acid, methanol, and phosphoric acid. Statistical Analysis  This was done using R Studio (version 4.2.0). Results  The study found that the UV-HPLC method utilizing formic acid (0.05–0.1%): acetonitrile in a 1:9 as mobile phase was the most effective for measuring 5-HIAA in human CSF. The method exhibited excellent linearity, accuracy, and precision. Conclusion  The study concludes that the developed UV-HPLC method is reliable and effective for measuring 5-HIAA in human CSF. Measuring 5-HIAA levels in CSF can serve as a potential biomarker for neurological and psychiatric illnesses, including depressive disorders with suicidal behavior. This method is promising for clinical and forensic practice to diagnose suicidal cases. Further research is needed to determine the clinical significance of these findings and the potential for broader application in psychiatry. This article helps to give a practical, cost-effective methodology to detect CSF 5-HIAA.
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来源期刊
Journal of Laboratory Physicians
Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-
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发文量
99
审稿时长
31 weeks
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