大戟科猕猴的化学成分及其抗氧化作用

Q4 Multidisciplinary ASM Science Journal Pub Date : 2023-01-30 DOI:10.32802/asmscj.2023.1046
Aisyah Salihah Kamarozaman
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引用次数: 0

摘要

Macaranga属在世界范围内约有300种,普遍分布于非洲、东南亚、中国和印度的热带地区。据报道,该属植物具有多种药用特性,传统上用于治疗各种疾病,如真菌感染、疟疾、胃痛和腹泻。该属还含有许多酚类化合物,特别是烯酰化类黄酮和二苯乙烯,其次是萜类和单宁。本文利用多种色谱分离技术对马卡兰树皮粗丙酮提取物进行了植物化学研究,并利用多种光谱方法对分离化合物的结构进行了表征。取黄皮粉(2.05 kg),室温丙酮浸泡24小时,重复3次。粗提物(38.8 g)溶于甲醇(200 mL)中,用乙醚分馏,得到单宁含量较少的粗提物(10.9 g)。粗提物经真空液相色谱(VLC)得到9个半纯化馏分(ML1-9)。对馏分ML2 (448 mg)进行分离纯化,得到化合物1 (6.2 mg)和化合物3 (0.3 mg)。分离物ML7 (220 mg)经径向层析(RC)得到纯化合物2 (2.1 mg)。利用核磁共振(NMR)、紫外-可见(UV-Vis)、红外(IR)和质谱(MS)对化合物进行了鉴定,并与文献进行了比较。两种酚类化合物,即戊酸阿魏酸酯(1)和东莨菪碱(2),以及一种甾醇,称为豆甾醇(3),成功地从M. lowii茎皮纯化。东莨菪素(2)对DPPH自由基的清除活性中等,IC50值为34.82 mM。
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Chemical Constituents from Macaranga lowii King ex Hook.f. (Euphorbiaceae) and Their Effects on Antioxidant Activity
The genus Macaranga comprises about 300 species worldwide and is commonly distributed in the tropical region of Africa, Southeast Asia, China and India. The plant of this genus was reported to possess various medicinal properties which have been traditionally used to treat various illnesses such as fungal infection, malaria, stomachache, and diarrhoea. This genus also contains many phenolic compounds, particularly prenylated flavonoids and stilbenoids, followed by terpenoids and tannins. The phytochemical study on the crude acetone extract from the stem bark of Macaranga lowii was carried out to isolate chemical constituents using several chromatographic techniques and characterise the structure of the isolated compounds using various spectroscopic methods. The powdered stem bark of M. lowii (2.05 kg) was macerated in acetone for 24 hours at room temperature and repeated three times. The crude extract (38.8 g) was dissolved in methanol (200 mL) and fractionated with diethyl ether to reduce tannin and yield a crude extract with less tannin (10.9 g). The crude extract was subjected to vacuum liquid chromatography (VLC) to give nine semi-purified fractions (ML1-9). Fractionation and purification of fraction ML2 (448 mg) yielded compound 1 (6.2 mg) and 3 (0.3 mg). Fraction ML7 (220 mg) was subjected to radial chromatography (RC) to give a pure compound 2 (2.1 mg). The compounds were elucidated using Nuclear Magnetic Resonance (NMR), Ultraviolet-Visible (UV-Vis), Infrared (IR) and Mass spectrometry (MS), as well as comparison with the previous literature. Two phenolic compounds namely pentadecyl ferulate (1) and scopoletin (2) together with a sterol known as stigmasterol (3) were purified successfully from the stem bark of M. lowii. Scopoletin (2) exhibited moderate activity against DPPH radical scavenging with the IC50 value of 34.82 mM.
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ASM Science Journal
ASM Science Journal Multidisciplinary-Multidisciplinary
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0.60
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23
期刊介绍: The ASM Science Journal publishes advancements in the broad fields of medical, engineering, earth, mathematical, physical, chemical and agricultural sciences as well as ICT. Scientific articles published will be on the basis of originality, importance and significant contribution to science, scientific research and the public. Scientific articles published will be on the basis of originality, importance and significant contribution to science, scientific research and the public. Scientists who subscribe to the fields listed above will be the source of papers to the journal. All articles will be reviewed by at least two experts in that particular field.
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