鹿茸对环磷酰胺免疫抑制小鼠免疫活性的影响

IF 1.7 3区农林科学 Q3 CHEMISTRY, APPLIED Food and Agricultural Immunology Pub Date : 2022-10-10 DOI:10.1080/09540105.2022.2128070

Liu Song-xin, Li Zhi-man, Sha Zi-Jun, Xia Yun-shi, Zhao Li-juan, Ren Duo-duo, Sun Yin-shi

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引用次数: 1

摘要

摘要为了研究鹿茸（VA）对环磷酰胺诱导的免疫功能低下小鼠的抗氧化和免疫保护活性，对BALB/c小鼠进行了100、300或500次灌胃给药 mg/（kg·d）VA持续30天，并腹腔注射环磷酰胺以建立低免疫小鼠模型。分析了细胞免疫、巨噬细胞吞噬活性、自然杀伤细胞活性、白细胞计数、脾淋巴细胞亚群和血清细胞因子mRNA表达水平。我们发现，去除VA的2，2’-叠氮基-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）、OH和1，1-二苯基-2-苦基肼（DPPH）自由基的IC50分别为0.476、4.33和37.92 mg/mL。VA可通过提高细胞免疫、巨噬细胞吞噬能力和NK细胞活性来增强小鼠的先天免疫和适应性免疫；促进IL-4、IFN-γ、TNF-α等细胞因子的产生；增加淋巴细胞亚群的含量和比例。这些结果表明，VA具有良好的抗氧化活性，可以改善环磷酰胺诱导的免疫缺陷小鼠的免疫功能。

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Effect of velvet antler on the immune activity of cyclophosphamide-induced immunosuppressed mice

ABSTRACT To study the antioxidant and immune protection activity of velvet antler (VA) in cyclophosphamide-induced immunocompromised mice, BALB/c mice were intragastrically administered 100, 300, or 500 mg/(kg·d) VA for 30 days, and were intraabdominally injected with cyclophosphamide to create a hypoimmune murine model. Cellular immunity, macrophage phagocytic activity, natural killer (NK) cell activity, white blood cell count, splenic lymphocyte subsets and the mRNA expression levels of serum cytokines were analysed. We found that the IC50 for removing 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), OH, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals of VA were 0.476, 4.33, and 37.92 mg/mL, respectively. VA could enhance innate and adaptive immunity of mice by improving cellular immunity, macrophage phagocytic ability, and NK cell activity; promoting the production of IL-4, IFN-γ, TNF-α and other cytokines; increasing the content and ratio of lymphocyte subsets. These results indicate that VA has good antioxidant activity and can improve the immune function of cyclophosphamide-induced immunodeficient mice.

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来源期刊

Food and Agricultural Immunology 农林科学-毒理学

CiteScore

5.30

自引率

6.70%

发文量

审稿时长

2 months

期刊介绍： Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed. The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on: -Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer -Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals -Effects of food on the immune system -Studies on allergy and allergic reactions -Investigations into food allergies -Development of allergen-free food systems -Development of novel assay formats -Applications of assay systems to the monitoring of food items in relation to safety and labelling -Food quality issues, e.g. speciation, adulteration and contamination -Comparisons between different analytical techniques The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.