特朗花提取物对白色念珠菌生长的抗菌试验

Anas fadli Wijaya, Rian anggia Destiawan, Nosa Ika Cahyariza
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引用次数: 0

摘要

念珠菌感染是由念珠菌群,尤其是白色念珠菌引起的一种急性和亚急性真菌感染。由微生物引起的传染病可以用现代药物治疗,即抗真菌药物。在某些情况下,抗菌药物往往由于给药不当而产生耐药性。这种耐药性的出现鼓励了对可作为抗菌替代品的新活性成分的进一步研究,特别是从含有类黄酮的植物来源中。本研究的目的是测定特朗花70%乙醇提取物中抗菌成分对白色念珠菌生长的影响。本研究是一项仅采用试验后对照组设计方法的实验室实验研究。抗菌活性的测定是通过点蚀法进行的。用10%DMSO稀释的特朗花提取物,取30µl,然后滴入井中。使用抗微生物酮康唑的阳性对照和使用滴在井上的10%二甲基亚砜的阴性对照多达30µl。根据观察结果,仅在阳性对照中形成了平均15.5+0.4mm的抑制区。已经进行的70%的特朗花的乙醇提取物的结果没有显示出对真菌生长的白色念珠菌的抗菌活性。该抑制区的缺失是由于特朗花中负责抗菌的活性化合物浓度未知,因此有必要进行研究,寻找具有抗菌能力的活性化合物的浓度。
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Antimicrobial Test of Telang Flower Extract (Clitoria ternatea L.) against the Growth of Candida albicans
Candidiasis is an acute and subacute fungal infection caused by the Candida group, especially Candida albicans. Infectious diseases due to microbes can be treated using modern drugs, namely antifungals. In some cases, antimicrobials often develop resistance due to improper administration. The occurrence of such resistance encourages further research on new active ingredients that can be used as antimicrobial alternatives, especially from plant sources containing flavonoids. The purpose of this study is to determine the effect of antimicrobial content of ethanol extract 70% of Telang flowers on the growth of C. albicans. This research is a laboratory experimental research with post test control group design only method. Determination of antimicrobial activity is carried out by the pitting method. Telang flower extract that has been diluted with 10% DMSO, taken as much as 30µl then dripped into the well. Positive control using antimicrobial ketocenazole and negative control using DMSO 10% which was dripped on the well as much as 30µl. Based on the results of observations, the inhibition zone is only formed in positive control with an average of 15.5 + 0.4 mm. The results of ethanol extract of 70% Telang flowers that have been conducted did not show antimicrobial activity against fungal growth C. albicans. The absence of this inhibitory zone is due to the unknown concentration of active compounds of Telang flowers that are responsible as antimicrobials, so it is necessary to conduct research on the search for concentrations of active compounds that have the ability as antimicrobials.
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