葡萄藤灰比诺病毒感染罗马尼亚的葡萄藤-简短通讯

IF 1 Q3 HORTICULTURE Horticultural Science Pub Date : 2021-03-31 DOI:10.17221/65/2020-HORTSCI
I. Guţă, E. Buciumeanu
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引用次数: 3

摘要

自2012年以来,世界上许多葡萄种植国都发现了葡萄灰皮诺病毒(GPGV)。这项工作的目的是调查从种质收集的一些材料上,除了繁殖材料和克隆选择样本外,GPGV的存在。在2019年至2020年期间,共有199个样本通过双抗体夹心酶联免疫吸附测定法(DAS-ELISA)分析了GPGV、葡萄扇叶病毒(GFLV)、葡萄卷叶相关病毒1+3(GLRaV-1+3)和葡萄斑点病毒(GFkV)的存在。其中,107个样本(53.76%)显示GPGV感染,与叶片(变形、黄化、马赛克、皱纹)或发育迟缓的植物症状相关或无症状。感染品种分布以老品种感染率高(37.38%),其次为无性系(32.71%)、砧木(11.21%)、无性系选择(9.35%)和新品种(93.5%),发现GPGV与GFkV(5例)和GLRaV-1+3(2例)有关。GPGV应被纳入葡萄藤无病毒母株生产认证计划的规则中。
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Grapevine Pinot gris virus infecting grapevines in Romania - Short Communicaiton
Grapevine Pinot gris virus (GPGV) has been identified in many grape growing countries of the world since 2012. The aim of this work was to investigate the presence of GPGV on some accessions collected from a germplasm collection, in addition to the propagation material and clonal selection samples. During 2019–2020, a total of 199 samples have been analysed by a double antibody sandwich – enzyme-linked immunosorbent assay (DAS-ELISA) for the presence of GPGV, Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus-1+3 (GLRaV-1+3) and Grapevine fleck virus (GFkV). Among them, 107 samples (53.76%) showed a GPGV-infection, associated with or without symptoms on the leaves (deformations, chlorosis, mosaic, wrinkles) or stunting plants. The distribution of infected varieties showed a high rate of infection in old varieties (37.38%), followed by clones (32.71%), rootstocks (11.21%), clonal selections (9.35%) and new varieties (9.35%). The tests revealed the association of GPGV with GFkV (5 cases) and GLRaV-1+3 (2 cases). GPGV should be included in the rules of grapevine certification schemes for the production of virus-free mother plants.
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来源期刊
Horticultural Science
Horticultural Science Horticulture-园艺
CiteScore
2.20
自引率
0.00%
发文量
23
审稿时长
>12 weeks
期刊介绍: The journal publishes results of basic and applied research from all areas of horticulture, fruit-growing, vegetable-growing, wine-making and viticulture, floriculture, ornamental gardening, garden and landscape architecture, concerning plants that are grown under the conditions of European temperate zone, or field plants that are considered as horticultural cultures. Original scientific papers, short communications and review articles are published in the journal. Papers are published in English (British spelling).
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