阿根廷布宜诺斯艾利斯省近海仿射亚历山Alexandrium affine (Dinophyceae)的形态、分子和毒理学分析

IF 1.5 4区 生物学 Q3 MARINE & FRESHWATER BIOLOGY Phycologia Pub Date : 2023-07-04 DOI:10.1080/00318884.2023.2223796
J. A. Tardivo Kubis, F. Rodríguez, A. Rossignoli, P. Riobó, Delfina Aguiar Juárez, E. Sar, Inés Sunesen
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引用次数: 0

摘要

摘要在布宜诺斯艾利斯省海洋沿海水域有害微藻监测项目的框架内,分离出一株仿射亚历山大藻(LPCc012),以减轻有害藻华对水产养殖和海洋生物的影响,保护人类健康。仿射亚历山大藻(Alexandrium affine)是一种分布在世界各地的有害藻华形成物种,具有产生毒素和非毒素的菌株。使用光学和扫描电子显微镜对布宜诺斯艾利斯省的田间和培养材料进行了检查,对其进行了形态学和遗传学(LSU rDNA部分测序)表征,并与世界各地的材料进行了比较。使用荧光检测的超高效液相色谱法和液相色谱-质谱法进行的毒素分析未能检测到任何麻痹性或亲脂性贝类毒素。这是来自阿根廷的A.affine的第一个记录。
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Morphological, molecular and toxinological analyses of Alexandrium affine (Dinophyceae) from marine coastal waters of Buenos Aires Province (Argentina)
ABSTRACT In the framework of a monitoring program of harmful microalgae from the marine coastal waters of Buenos Aires Province to mitigate the impacts of harmful algal blooms on the aquaculture and marine life and to protect human health, a strain of Alexandrium affine (LPCc012) was isolated. Alexandrium affine is a worldwide distributed species reported as harmful algal bloom-forming, with toxin- and non-toxin-producing strains. Field and cultured materials from Buenos Aires Province were examined using light and scanning electron microscopy, morphologically and genetically (LSU rDNA partial sequencing) characterized, and compared with material from different regions worldwide. Toxin analyses using ultra-performance liquid chromatography with fluorescence detection and liquid chromatography-mass spectrometry methods failed to detect any paralytic or lipophilic shellfish toxins. This is the first record of A. affine from Argentina.
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来源期刊
Phycologia
Phycologia 生物-海洋与淡水生物学
CiteScore
3.10
自引率
18.80%
发文量
54
审稿时长
3 months
期刊介绍: Phycologia is published bimonthly by the International Phycological Society and serves as a publishing medium for information about any aspect of phycology. Membership in the Society is not necessary for publication. Submitted manuscripts cannot be previously published or submitted elsewhere. Copyright ownership of all accepted papers is held by the International Phycological Society.
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