美国德克萨斯州南部麻蝇(腹足目:麻蝇科)13个多态微卫星位点的开发、鉴定与利用

IF 0.4 4区生物学 Q4 MARINE & FRESHWATER BIOLOGY American Malacological Bulletin Pub Date : 2017-11-14 DOI:10.4003/006.035.0208

Russell L. Minton, Rochelle R. Beasley, S. Lance, Kathryn E. Perez

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摘要

摘要/ Abstract摘要:在南德克萨斯种Praticolella von Martens, 1892(腹足目:多角蝽科)中开发并鉴定了13个多态微卫星位点，其中一些位点分布受限，难以用传统的壳性状进行鉴定。为了使这个基因组工具在这些物种中更广泛地应用，我们不是在单个物种的许多个体中表征位点，而是在四个不同物种的五个种群中进行了测试。在所有4个被测物种中有12个位点扩增，11个位点偏离Hardy-Weinberg平衡。这些基因座对评估南德克萨斯鸡的物种特性和种群遗传连通性具有重要价值。

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Development, Characterization, and Utility of 13 Polymorphic Microsatellite Loci in Praticolella (Gastropoda: Polygyridae) Species from South Texas, U.S.A.

Abstract: We developed and characterized 13 polymorphic microsatellite loci in South Texas species of Praticolella von Martens, 1892 (Gastropoda: Polygyridae), some of which have restricted distributions and can be difficult to identify using traditional shell characters. To make this genomic tool more broadly useful in these species, instead of characterizing loci in many individuals of a single species, we tested them across five populations of four different species. Twelve loci amplified in all four species tested, and eleven loci showed deviations from Hardy-Weinberg equilibrium. These loci will be valuable in assessing species identity and population genetic connectivity among South Texas Praticolella.

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来源期刊

American Malacological Bulletin 生物-动物学

CiteScore

1.00

自引率

40.00%

发文量

审稿时长

>12 weeks

期刊介绍： The American Malacological Bulletin serves as an outlet for reporting notable contributions in malacological research. Manuscripts concerning any aspect of original, unpublished research,important short reports, and detailed reviews dealing with molluscs will be considered for publication. Recent issues have included AMS symposia, independent papers, research notes,and book reviews. All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymous reviewing by independent expertreferees. AMS symposium papers have undergone peer review by symposium organizer, symposium participants, and independent referees.