以杏心尖节为外植体的杏品种离体繁殖

4区 医学 Q4 Agricultural and Biological Sciences Progress in Nutrition Pub Date : 2018-08-06 DOI:10.23751/PN.V20I1-S.5576
F. A. Özdemir, N. Gur
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引用次数: 4

摘要

杏(Prunus armeniaca L.)是一种营养丰富的水果,杏植株的微繁栽培具有很高的经济价值。本研究报道了品种Cataloglu的根尖外植体在Murashige和Skoog (MS)培养基上的微繁殖,该培养基含有添加3%蔗糖的不同BAP+NAA组合,并用0.65%琼脂固化。结果表明,不同浓度的BAP+NAA对愈伤组织诱导、芽再生和芽长均有显著影响。在不同浓度的BAP+NAA处理下,所有再生苗的生根率均不受影响。然而,每个外植体的根数和根长与BAP+NAA的浓度有明显的关系。在含1mg /l BAP + 0.25 mg/l NAA的MS培养基上再生效果最佳;在含1mg /l IBA的MS培养基上生根。在温室条件下,将生根苗置于土沙比(3:1)的盆栽中进行驯化。本研究可为今后杏的育种研究和遗传转化研究提供参考。
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In vitro propagation of Cataloglu Apricot (Prunus armeniaca L.) cultivar using apical node as explant
Apricot (Prunus armeniaca L.) is highly nutritious fruit and micro propagation of apricot plant has high economic value. The current study reports micro propagation of cultivar Cataloglu using apical node explants on Murashige and Skoog (MS) medium containing different combinations of BAP+NAA supplemented with 3% sucrose and solidified with 0.65% agar. The results showed a significant effect of concentrations of BAP+NAA on callus induction, shoot regeneration and their length. Rooting percentage was not affected on all shoots regenerated on any concentration of BAP+NAA. However, number of roots per explants and their length had clear implications of the concentrations of BAP+NAA used for regenerating shoots. Optimum shoot regeneration was noted on MS medium containing 1 mg/l BAP + 0.25 mg/l NAA; which were rooted on MS medium containing 1 mg/l IBA. The rooted plantlets were acclimatized in pots containing soil:sand (3:1) ratio under greenhouse conditions. It is concluded that the study will serve as reference for future apricot breeding studies and genetic transformation studies.
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来源期刊
Progress in Nutrition
Progress in Nutrition 医学-营养学
CiteScore
1.40
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Progress in Nutrition was founded in 1999 as an independent magazine, a multidisciplinary approach, dedicated to issues of nutrition and metabolism.
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