M. Mousavi, A. E. Nejad, E. Shamsoddin, M. M. Golabgiran, B. Houshmand
{"title":"沸石作为骨生物改性载体的体外研究","authors":"M. Mousavi, A. E. Nejad, E. Shamsoddin, M. M. Golabgiran, B. Houshmand","doi":"10.5539/jmbr.v9n1p126","DOIUrl":null,"url":null,"abstract":"Background: Zeolite is a microporous aluminosilicate compound which has been successfully used in tissue engineering. The effects of Zeolite on the morphology and functions of pre-osteoblastic MG-63 cells as new bone enhancer material is still unclear. \n \nMethods: In this vitro experimental study, MTT and Alizarin red staining test were performed on six groups of MG-63 cells which differed in Zeolite (Z) concentration and the presence or absence of Alloplast extract (A). Group A: 0.1μg/mL Z+A, Group B: 0.1μg/mL Z without A, Group C: 0.2μg/mL Z+A, Group D: 0.2μg/mL Z without A, Group E: 0.3μg/mL Z+A, Group F: 0.3μg/mL Z without A. There were also three control groups as positive control, negative control, and Alloplast control based on each related test. The data were analyzed by SPSS 20 via one-way ANOVA and Welch test. (P<0.05). \n \nResults: At 24 hours, results showed that solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p<0.001). At 72hours time point, the results showed significantly higher proliferation rates in the solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast compared to the positive control group without Alloplast (p<0.001). \n \nConclusions: Zeolite can increase proliferation of MG-63 cells without presence of Alloplast; It seems that combination of Zeolite with Alloplast maybe enhancing proliferation and function of MG-63 cells.","PeriodicalId":92078,"journal":{"name":"Journal of molecular biology research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Zeolite as a Bone Bio-Modifier Carrier: An In-Vitro Study\",\"authors\":\"M. Mousavi, A. E. Nejad, E. Shamsoddin, M. M. Golabgiran, B. Houshmand\",\"doi\":\"10.5539/jmbr.v9n1p126\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Zeolite is a microporous aluminosilicate compound which has been successfully used in tissue engineering. The effects of Zeolite on the morphology and functions of pre-osteoblastic MG-63 cells as new bone enhancer material is still unclear. \\n \\nMethods: In this vitro experimental study, MTT and Alizarin red staining test were performed on six groups of MG-63 cells which differed in Zeolite (Z) concentration and the presence or absence of Alloplast extract (A). Group A: 0.1μg/mL Z+A, Group B: 0.1μg/mL Z without A, Group C: 0.2μg/mL Z+A, Group D: 0.2μg/mL Z without A, Group E: 0.3μg/mL Z+A, Group F: 0.3μg/mL Z without A. There were also three control groups as positive control, negative control, and Alloplast control based on each related test. The data were analyzed by SPSS 20 via one-way ANOVA and Welch test. (P<0.05). \\n \\nResults: At 24 hours, results showed that solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p<0.001). At 72hours time point, the results showed significantly higher proliferation rates in the solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast compared to the positive control group without Alloplast (p<0.001). \\n \\nConclusions: Zeolite can increase proliferation of MG-63 cells without presence of Alloplast; It seems that combination of Zeolite with Alloplast maybe enhancing proliferation and function of MG-63 cells.\",\"PeriodicalId\":92078,\"journal\":{\"name\":\"Journal of molecular biology research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-10-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of molecular biology research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5539/jmbr.v9n1p126\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of molecular biology research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5539/jmbr.v9n1p126","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Zeolite as a Bone Bio-Modifier Carrier: An In-Vitro Study
Background: Zeolite is a microporous aluminosilicate compound which has been successfully used in tissue engineering. The effects of Zeolite on the morphology and functions of pre-osteoblastic MG-63 cells as new bone enhancer material is still unclear.
Methods: In this vitro experimental study, MTT and Alizarin red staining test were performed on six groups of MG-63 cells which differed in Zeolite (Z) concentration and the presence or absence of Alloplast extract (A). Group A: 0.1μg/mL Z+A, Group B: 0.1μg/mL Z without A, Group C: 0.2μg/mL Z+A, Group D: 0.2μg/mL Z without A, Group E: 0.3μg/mL Z+A, Group F: 0.3μg/mL Z without A. There were also three control groups as positive control, negative control, and Alloplast control based on each related test. The data were analyzed by SPSS 20 via one-way ANOVA and Welch test. (P<0.05).
Results: At 24 hours, results showed that solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p<0.001). At 72hours time point, the results showed significantly higher proliferation rates in the solutions with 0.1μg/mL, 0.2μg/mL, and 0.3μg/mL Zeolite with or without Alloplast compared to the positive control group without Alloplast (p<0.001).
Conclusions: Zeolite can increase proliferation of MG-63 cells without presence of Alloplast; It seems that combination of Zeolite with Alloplast maybe enhancing proliferation and function of MG-63 cells.