Expression and significance of TNF-α and M-CSF in placenta tissue of early severe preeclampsia

Objective To investigate the expression and clinical significance of macrophage colony stimulating factor (M-CSF) and tumor necrosis factor α (TNF-α) in placenta of early onset severe preeclampsia (PE). Methods Immunohistochemical SP method was used to detect the expression of M-CSF and TNF-α in 77 cases paraffin specimens from Department of Pathology, First Affiliated Hospital of Henan University of Science and Technology from September 2015 to September 2017, including 35 cases of early-onset severe PE, 42 cases of late-onset severe PE and 30 cases of normal pregnant women. Results (1)The positive expression rates of M-CSF in control group, late-onset severe PE group and early-onset severe PE group were 30% (9/30), 61.90% (26/42), 82.86% (29/35), respectively.The difference was statistically significant (χ2=18.90, P<0.05). The positive expression rates of early-onset severe PE group were significantly higher than those in control group (χ2=18.59, P<0.05), and the difference was statistically significant..The positive expression of early-onset severe PE group was higher than that of late-onset severe PE group, and the difference was statistically significant (χ2=4.017, P<0.05). (2) The positive expression rates of TNF-α in control group, late-onset severe PE group and early-onset severe PE group were 33.33% (10/30), 69.05% (29/42), 91.43% (32/35), respectively.The difference was statistically significant (χ2=30.21, P<0.05). The positive expression of TNF-α in early-onset severe PE group was significantly higher than that in control group (χ2=21.37, P<0.05), and the difference was statistically significant.The positive expression of M-CSF and TNF-a in early-onset severe PE group was higher than that in late-onset severe PE group, and the difference was statistically significant (χ2=4.529, P<0.05); (3) The expression of M-CSF and TNF-α was positively correlated in PE (r=0.441, P=0.000). Conclusion Placental damage is higher in early-onset severe PE, and is related to the severity of the disease.The levels of M-CSF and TNF-alpha in placenta of PE patients may play a synergistic role in the occurrence and development of PE. Key words: Preeclampsia; Early onset severe preeclampsia; Placental tissue; Macrophage colony-stimulating factor; Tumor necrosis factor α