柠檬酸铵浓度变化对碳点粘菌素修饰检测大肠杆菌的影响

IF 2.4 Q3 CHEMISTRY, MULTIDISCIPLINARY Moroccan Journal of Chemistry Pub Date : 2021-08-07 DOI:10.48317/IMIST.PRSM/MORJCHEM-V9I3.23191
S. Suherman, Yoga Nurwijaya, E. Wahyuni, D. Siswanta, K. Morita, Y. Oki
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引用次数: 0

摘要

世界卫生组织(世界卫生组织)提到,腹泻是发展中国家因大肠杆菌(E.coli)细菌而导致的未成年人死亡的主要原因之一。以柠檬酸铵为前体合成粘菌素偶联碳点,用于大肠杆菌检测,研究其在城市人口水井水样检测中的应用。采用传统的热解法合成碳点。质量变化为10的柠檬酸铵;15;20;和25mg加入到2.5g粘菌素中并在180℃下加热持续1小时。得到棕色残留物;通过FT-IR和TEM对更多的官能团和形貌进行了表征。用蒸馏水进一步稀释棕色残留物,以8000rpm离心10分钟。使用分光光度计UV-Vis分析吸光度。在310-450nm处使用分光光度计荧光分析具有不同柠檬酸铵浓度变化的碳点粘菌素,最高强度将用于大肠杆菌检测。从实验部分发现,CDs粘菌素具有直径约3-10nm的球形形态。用20mg柠檬酸铵从CDs粘菌素在360nm的激发波长下获得最高强度。在对印度尼西亚日惹的三个家庭城市井水的检测过程中,得出的结论是,一个样本中含有大肠杆菌,其浓度高于印度尼西亚卫生部规定的每100毫升235 cfu的允许限值。
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Effect of ammonium citrate concentration variation on the carbon dots colistin modification for Escherichia coli detection
World Health Organization (WHO) mentioned that diarrhea is one of the main causes of under-five mortality in developing countries due to Escherichia coli ( E. coli ) bacteria. Synthesis of colistin-conjugated-carbon dots using ammonium citrate as a precursor for E. coli detection has been carried out to study its application for detection in water samples from urban population wells. Synthesis of carbon dots (CDs) was carrried out using conventional pyrolisis method. Ammonium citrate with mass variation of 10; 15; 20; and 25 mg were added to 2.5 g colistin and heated at 180 C for 1 h. Brown residue was yielded; more over the functional groups and morphology were characterized by FT-IR and TEM. The brown residue is further diluted by distilled water, centrifuged at 8000 rpm for 10 min. The absorbances were analyzed using spectrophotometer UV-Vis. Carbon dots colistin with different concentration of ammonium citrate variations were analyzed using spectrophotometer fluorescence at 310-450 nm and the highest intensity would be used for E. coli detection. From the experimental part, it was found that CDs colistin has spherical morphology with diameter around 3-10 nm. The highest intensity was achieved at excitation wavelength of 360 nm from CDs colistin with 20 mg ammonium citrate. In the detection process of three household urban wells water in Yogyakarta-Indonesia, it was concluded that one sample was found to contain E. coli whose concentration was higher than the permitted limit of 235 cfu per 100 mL set by Ministry of Health-Republic of Indonesia.
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来源期刊
Moroccan Journal of Chemistry
Moroccan Journal of Chemistry CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
3.40
自引率
9.10%
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0
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