{"title":"高效液相色谱- dad、ESI/MSn快速分析具有抗肿瘤作用的食药用药鹿茸的生物活性成分","authors":"Meng Zhang, Dong Liu, Yuqing Zhang","doi":"10.5073/JABFQ.2019.092.017","DOIUrl":null,"url":null,"abstract":"Saxifraga stolonifera is an edible and herbaceous plant, which has been demonstrated to have anti-tumor effects in vivo and in vitro. The aim of this paper is to determine the main bioactive components inS. stolonifera, and their distribution in different parts of S. stolonifera and in S. stolonifera that was cultivated in different places in China using a high-performance liquid chromatography-diode array detector and electrospray ionization/ion trap mass spectrometry (HPLC-DAD-ESI/MSn). Four main components were identified and three were quantified. The contents of gallic acid, protocatechuic acid and bergenin had significant differences not only between the roots and stems-leaves of the plant, but also among different cultivated varieties of S. stolonifera. The experiment showed that the method used here exhibited good repeatability and recovery. Therefore, the results provide reliable data for research and development in the future on the level and distribution of the three bioactive components of S. stolonifera.","PeriodicalId":56276,"journal":{"name":"Journal of Applied Botany and Food Quality-Angewandte Botanik","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2019-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Rapid analysis of the bioactive components in Saxifraga stolonifera, an edible and medicinal herb with anti-tumor effects, by HPLC-DAD, ESI/MSn\",\"authors\":\"Meng Zhang, Dong Liu, Yuqing Zhang\",\"doi\":\"10.5073/JABFQ.2019.092.017\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Saxifraga stolonifera is an edible and herbaceous plant, which has been demonstrated to have anti-tumor effects in vivo and in vitro. The aim of this paper is to determine the main bioactive components inS. stolonifera, and their distribution in different parts of S. stolonifera and in S. stolonifera that was cultivated in different places in China using a high-performance liquid chromatography-diode array detector and electrospray ionization/ion trap mass spectrometry (HPLC-DAD-ESI/MSn). Four main components were identified and three were quantified. The contents of gallic acid, protocatechuic acid and bergenin had significant differences not only between the roots and stems-leaves of the plant, but also among different cultivated varieties of S. stolonifera. The experiment showed that the method used here exhibited good repeatability and recovery. Therefore, the results provide reliable data for research and development in the future on the level and distribution of the three bioactive components of S. stolonifera.\",\"PeriodicalId\":56276,\"journal\":{\"name\":\"Journal of Applied Botany and Food Quality-Angewandte Botanik\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2019-05-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Botany and Food Quality-Angewandte Botanik\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.5073/JABFQ.2019.092.017\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Botany and Food Quality-Angewandte Botanik","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.5073/JABFQ.2019.092.017","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Rapid analysis of the bioactive components in Saxifraga stolonifera, an edible and medicinal herb with anti-tumor effects, by HPLC-DAD, ESI/MSn
Saxifraga stolonifera is an edible and herbaceous plant, which has been demonstrated to have anti-tumor effects in vivo and in vitro. The aim of this paper is to determine the main bioactive components inS. stolonifera, and their distribution in different parts of S. stolonifera and in S. stolonifera that was cultivated in different places in China using a high-performance liquid chromatography-diode array detector and electrospray ionization/ion trap mass spectrometry (HPLC-DAD-ESI/MSn). Four main components were identified and three were quantified. The contents of gallic acid, protocatechuic acid and bergenin had significant differences not only between the roots and stems-leaves of the plant, but also among different cultivated varieties of S. stolonifera. The experiment showed that the method used here exhibited good repeatability and recovery. Therefore, the results provide reliable data for research and development in the future on the level and distribution of the three bioactive components of S. stolonifera.
期刊介绍：
The Journal of Applied Botany and Food Quality is the Open Access journal of the German Society for Quality Research on Plant Foods and the Section Applied Botany of the German Botanical Society. It provides a platform for scientists to disseminate recent results of applied plant research in plant physiology and plant ecology, plant biotechnology, plant breeding and cultivation, phytomedicine, plant nutrition, plant stress and resistance, plant microbiology, plant analysis (including -omics techniques), and plant food chemistry. The articles have a clear focus on botanical and plant quality aspects and contain new and innovative information based on state-of-the-art methodologies.
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