Comparative evaluation of Glycyrrhiza glabra incorporated soft-liner over conventional soft-liner based on antimicrobial activity, anti-inflammatory efficacy, surface roughness, wettability, and tensile bond strength: An in vitro study

Aim: To determine the anti-inflammatory and antimicrobial activity of Glycyrrhiza glabra incorporated in a soft-liner that can be used as denture relining material. Materials and Methods: 3 g of the powdered G. glabra leaves were added to 100 mL of deionized water and filtered after boiling at 100°C for 10 min and the filtrate was boiled until it was reduced to 10 mL. The soft-liner liquid and G. glabra were then blended in proportions of 5 and 10 v/v%, and the mixtures were stirred for 24 h in a closed container. Sample preparation was done for surface roughness, contact angle, and antimicrobial activity: direct Glycyrrhiza solution was taken as a sample for an anti-inflammatory test. A well-diffusion method was performed to determine the antimicrobial effect. Anti-inflammatory activity was determined by inhibition of albumin denaturation assay, wettability through contact angle using a Telescopic Goniometer, surface roughness using a profilometer, and tensile bond strength by using UTM (Instron, ElectroPuls, E3000). All of the groups were compared by doing a one-way analysis of variance, an independent t test was used for comparing two experimental groups. Results: Statistically significant antibacterial property was noted (P <0.05). The maximum concentration of G. glabra’s anti-inflammatory activity was found to be 81% without altering its physical or mechanical properties (P > 0.05). Conclusion: Glycyrrhiza glabra incorporated soft-liner will be a better alternative of conventional softener.