中非某二线城市冈比亚按蚊复合体Kdr耐药基因及其空间分布:喀麦隆南部Ayos病例

Nopowo Nt, Akono Pn, N. R., Offono Le, Kojom R, Mache Pn, N. Oe, M. Ja, Mbongue Rs
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引用次数: 0

摘要

该研究于2019年12月和2020年2月在Ayos市Akoun(城市站点)和Ebabodo(城郊站点)两个地区进行,目的是研究冈比亚按蚊复合体成员的空间分布,确定其耐药性状况,并调查Kdr突变的发生和分布。采用浸渍法在幼虫期采集蚊虫,然后饲养至成虫期。成虫种群的易感性。根据世卫组织建议的议定书评估了冈比亚对滴滴涕和拟除虫菊酯类杀虫剂的影响。采用SINE PCR法对蚊虫进行鉴定。PCR检测Kdr突变时,仅检测幸存者。在研究地点,冈比亚复合体由An。coluzzi和An。冈比亚虫在产卵地同属生活,以安虫为主。coluzzii在Akoun(90.83%)和Ebabodo(76.69%)。用溴氰菊酯、氯菊酯和滴滴涕进行的试验显示,无论按蚊的原产地如何,死亡率都低于70%。Kdr突变的诊断性PCR显示,100%的幸存者在两个位点都有突变,两个物种的抗性等位基因频率为1.0。安的高电阻。coluzzi和An。冈比亚的杀虫剂需要开发新的杀虫分子。
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Kdr Resistance Gene and Spatial Distribution of Anopheles gambiae Complex Members in a Secondary City in Central Africa: Ayos Case (South Cameroon)
The study was conducted in December 2019 and February 2020 in two areas of Ayos city, Akoun (urban site) and Ebabodo (peri-urban site), in order to study the spatial distribution of members of the Anopheles gambiae complex, to determine their resistance status and to investigate the occurrence and distribution of the Kdr mutation. Mosquitoes were collected at the larval stage using the dipping method and then reared to the adult stage. The susceptibility of adult populations of An. gambiae s.l. to DDT and pyrethroids was assessed according to the WHO recommended protocol. Mosquitoes from the tests were identified by SINE PCR. Only test survivors were used for Kdr mutation testing by PCR. In the study sites, the gambiae complex was composed of An. coluzzii and An. gambiae living in sympatry in their oviposition sites with a predominance of An. coluzzii in Akoun (90.83%) and Ebabodo (76.69%). Tests with deltamethrin, permethrin and DDT revealed mortality rates of less than 70% whatever the locality of origin of the anopheles. Diagnostic PCR for the Kdr mutation showed that 100% of the survivors had the mutation in both sites, with frequencies of the resistant allele of 1.0 in both species. The high resistance of An. coluzzii and An. gambiae to insecticides requires the development of new insecticidal molecules.
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