K. Hachem, C. Faugeron-Girard, M. Kaid-Harche, V. Gloaguen
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引用次数: 5
摘要
摘要采用顺序碱萃取法从argan (Argania spinosa)叶片中提取木聚糖多糖，并用Sepharose CL-4B层析纯化木聚糖多糖。所得片段在Biogel P2上通过凝胶过滤分离。然后用气液色谱(GLC)和基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)对所得的低聚糖组分进行了表征。结果表明，多糖中存在由木糖和4- o -甲基葡萄糖醛酸组成的酸性寡糖基序，以及由鼠李糖和半乳糖醛酸组成的酸性寡糖基序。
Acid hydrolysis of xylan polysaccharides fractions isolated from argan (Argania spinosa) leaves
Abstract Xylan polysaccharides previously obtained from argan (Argania spinosa) leaves by sequential alkaline extractions and purified by chromatography on Sepharose CL-4B were fragmented by acid hydrolysis in 2 M HCl. The resulting fragments were separated by gel filtration on Biogel P2. The oligosaccharide fractions obtained were then characterized by gas–liquid chromatography (GLC) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The results show the presence of acidic oligosaccharide motifs, some of which composed of xylose and 4-O-methylglucuronic acid and other ones comprising rhamnose and galacturonic acid.
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