C. Castañeda, Miluska Castillo, Juvenal Sanchez, S. Casavilca, C. Gonzalez, C. Flores, Luis Cano, Carolina Belmar-López, Rosario Villa-Robles, Gabriela Rios-Martini, Yun Wu
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Factors influencing Ki67 calculation in neuroendocrine neoplasia
Aim: We compared different methodologies to evaluate Ki67 in neuroendocrine neoplasia (NEN). Patients & methods: ki67 was evaluated using three methods in 70 NEN cases: manual-count, eyeballed estimate made by three pathologists and ImmunoRatio-software. Manual-count was the standard method and agreement with it was evaluated through intraclass correlation (ICC). Results: Agreement between manual-count and eyeballed estimate had ICC: 0.887–0.929. Eyeballed estimate by three pathologists produced upgradation in 5.7–32.9% and downgradation in 5.7% of NEN cases. Agreement for ImmunoRatio-count had ICC: 0.989. Immunoratio produced upgradation in 17.1% and downgradation in 8.5% cases. Agreement between all methods was higher at low-Ki67. Cellularity, immune-cell infiltration, staining and sample quality did not affect agreement. Conclusion: Eyeballed estimate and ImmunoRatio showed good accuracy, especially at low-Ki67.
期刊介绍：
International Journal of Endocrine Oncology is a quarterly, peer-reviewed journal that helps the clinician to keep up to date with best practice in this fast-moving field. The journal highlights significant advances in basic and translational research, and places them in context for future therapy. The journal presents the latest research findings in diagnosis and management of endocrine cancer, together with authoritative reviews, cutting-edge editorials and perspectives that highlight hot topics and controversy in the field. Independent drug evaluations assess newly approved medications and their role in clinical practice. The journal welcomes the unsolicited submission of article proposals and original research manuscripts.
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