胰腺切除术后胰岛分离。适应症、方法、结果评价

V. Zagainov, D. Kuchin, P. Ermakova, E. A. Vasilchikova, L. A. Lugovaia, N. Naraliev, A. V. Kashina
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引用次数: 0

摘要

目标开发并实施一种用自体胰岛移植从胰腺切除术后的胰腺组织中分离胰岛的方法。材料和方法。该研究使用了10个Wiesenau微型猪胰腺、30个Wistar大鼠胰腺、8个切除的人类胰腺和5个大面积切除或胰腺切除的人类胰。后果获得了完全没有人类和实验动物腺泡组织的郎格罕群岛。当使用该方案进行机械隔离时,在人类和实验动物中隔离胰岛的效率为50%。研究证明,分离出的细胞属于朗格汉斯岛。此外,还确认了它们的纯度。分离的胰岛存活率超过90%。胰岛素合成的酶免疫测定显示,分离的朗格汉斯胰岛保持功能活性。已确认分离的胰岛细胞的无菌性。结论这种经过验证的分离技术使获得适合进一步自体移植的纯净、无菌、有活力和功能活性的胰岛细胞成为可能。
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Islet isolation from the pancreas after pancreatectomy. Indications, methodology, evaluation of results
Aim. To develop and implement a method for isolating the islets of Langerhans from pancreatic tissue after pancreatectomy with islet autotransplantation.Materials and Methods. The study used ten Wiesenau miniature pig pancreases, 30 Wistar rat pancreases, eight resected human pancreases, and five human pancreases from extensive resection or pancreatectomy.Results. Islets of Langerhans completely devoid of human and experimental animals’ acinar tissue were obtained. When using the protocol for mechanical isolation, the efficiency of isolating the islets of Langerhans in humans and experimental animals was 50%. The research proved that the isolated cells belonged to the islets of Langerhans. Additionally, their purity was confirmed. The survival of the isolated islets exceeded 90%. The enzyme immunoassay for insulin synthesis showed that the isolated islets of Langerhans remained functionally active. The sterility of the isolated islet cells was confirmed.Conclusion. This proven isolation technology makes it possible to obtain pure, sterile, viable, and functionally active islet cells suitable for further autotransplantation.
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来源期刊
Annals of HPB Surgery
Annals of HPB Surgery Medicine-Gastroenterology
CiteScore
0.70
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发文量
41
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