LOW LEVEL LASER THERAPY REDUCES ANTICANCER POTENTIAL OF COLLOIDAL SILVER THROUGH TRPV1 CHANNELS

Recently there are studies which particularly investigated efficiency of silver on neoplastic diseases. and many other diseases. Low level laser therapy(LLLT) has been shown to modulate biological processes and there are many studies that investigate the effect of LLLT, which is used in the treatment of cancers currently. Methods: Breast(MCF7) and colon(Caco 2) cells were cultured and cells were divided into eleven main groups. Cells were incubated with colloidal silver for 24 hrs. Colloidal silver and LLLT exposure separately and together performed on MCF7 and Caco 2 cell cultures. The effects of colloidal silver and LLLT were invastigated on all molecular pathways of apoptosis. Results: It was found that the levels of apoptosis in colloidal silver group were significantly increased in both cancer cells compared to control group. In both cancer cells, transient receptor protein vanilloid 1 (TRPV1) channel stimulator Cpsin administration resulted in significantly increased apoptosis levels compared to the control group, in colloidal silver + LLLT group the apopitosis level significantly reduced compared to colloidal silver-only group.Conclusions: It has been shown that colloidal silver significantly increases apoptosis in breast and colon cancer cells, however LLLT administration decreases apoptosis in both wavelength and suppressed apoptotic effect of colloidal silver significantly on these cells.