Nidal Naceiri Mrabti, H. N. Mrabti, L. Doudach, Zineb Khalil, M. R. Kachmar, Mouna Mekkaoui, M. Faouzi, E. Abdallah, G. Zengin, A. Bouyahya, M. Elhallaoui
{"title":"摩洛哥百里香水提取物的矿物质含量、抗菌特性、对啮齿类动物的急性和慢性毒性","authors":"Nidal Naceiri Mrabti, H. N. Mrabti, L. Doudach, Zineb Khalil, M. R. Kachmar, Mouna Mekkaoui, M. Faouzi, E. Abdallah, G. Zengin, A. Bouyahya, M. Elhallaoui","doi":"10.21448/ijsm.1106820","DOIUrl":null,"url":null,"abstract":"Moroccan flora is rich in plants used in traditional medicine, but the further scientific investigation is necessary. The aim of the research was to evaluate the nutritional content and antimicrobial activity of Moroccan Thymus vulgaris, as well as its possible acute and chronic toxicological effects on rodents. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) was used to determine the mineral content. The antimicrobial activity was determined using a well-diffusion test, a minimum inhibitory concentration (MIC), and a minimum bactericidal/fungicidal concentration (MBC/MFC) assay. Acute and chronic toxicity studies were conducted in vivo on mice and rats, respectively. Following that, haematological, serum-biochemistry, and histological investigations were performed. Moroccan Thyme was shown to be a source of numerous minerals which are necessary for health promotion. All antimicrobial testing, disc diffusion, MIC, and MBC tests revealed that thyme had potent antibacterial activity against all microorganisms tested. Staphylococcus aureus was the most susceptible bacterium, followed by Salmonella enterica and Escherichia coli. Additionally, thyme exhibited great antifungal efficacy against Candida albicans. The acute toxicity results indicated that the aqueous extract of T. vulgaris is almost non-toxic when taken orally. According to the chronic toxicity study, the extract is generally safe when taken orally over an extended period of time. The biochemical and haematological characteristics of the serum and blood were within acceptable limits, and histological examination revealed no abnormalities. In conclusion, the findings of this investigation, confirm the antimicrobial efficacy of the aqueous extract of Moroccan T. vulgaris and its safety for experimental animals.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mineral contents, antimicrobial profile, acute and chronic toxicity of the aqueous extract of Moroccan Thymus vulgaris in rodents\",\"authors\":\"Nidal Naceiri Mrabti, H. N. Mrabti, L. Doudach, Zineb Khalil, M. R. Kachmar, Mouna Mekkaoui, M. Faouzi, E. Abdallah, G. Zengin, A. Bouyahya, M. Elhallaoui\",\"doi\":\"10.21448/ijsm.1106820\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Moroccan flora is rich in plants used in traditional medicine, but the further scientific investigation is necessary. The aim of the research was to evaluate the nutritional content and antimicrobial activity of Moroccan Thymus vulgaris, as well as its possible acute and chronic toxicological effects on rodents. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) was used to determine the mineral content. The antimicrobial activity was determined using a well-diffusion test, a minimum inhibitory concentration (MIC), and a minimum bactericidal/fungicidal concentration (MBC/MFC) assay. Acute and chronic toxicity studies were conducted in vivo on mice and rats, respectively. Following that, haematological, serum-biochemistry, and histological investigations were performed. Moroccan Thyme was shown to be a source of numerous minerals which are necessary for health promotion. All antimicrobial testing, disc diffusion, MIC, and MBC tests revealed that thyme had potent antibacterial activity against all microorganisms tested. Staphylococcus aureus was the most susceptible bacterium, followed by Salmonella enterica and Escherichia coli. Additionally, thyme exhibited great antifungal efficacy against Candida albicans. The acute toxicity results indicated that the aqueous extract of T. vulgaris is almost non-toxic when taken orally. According to the chronic toxicity study, the extract is generally safe when taken orally over an extended period of time. The biochemical and haematological characteristics of the serum and blood were within acceptable limits, and histological examination revealed no abnormalities. In conclusion, the findings of this investigation, confirm the antimicrobial efficacy of the aqueous extract of Moroccan T. vulgaris and its safety for experimental animals.\",\"PeriodicalId\":14437,\"journal\":{\"name\":\"International Journal of Secondary Metabolite\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Secondary Metabolite\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21448/ijsm.1106820\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Secondary Metabolite","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21448/ijsm.1106820","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Mineral contents, antimicrobial profile, acute and chronic toxicity of the aqueous extract of Moroccan Thymus vulgaris in rodents
Moroccan flora is rich in plants used in traditional medicine, but the further scientific investigation is necessary. The aim of the research was to evaluate the nutritional content and antimicrobial activity of Moroccan Thymus vulgaris, as well as its possible acute and chronic toxicological effects on rodents. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) was used to determine the mineral content. The antimicrobial activity was determined using a well-diffusion test, a minimum inhibitory concentration (MIC), and a minimum bactericidal/fungicidal concentration (MBC/MFC) assay. Acute and chronic toxicity studies were conducted in vivo on mice and rats, respectively. Following that, haematological, serum-biochemistry, and histological investigations were performed. Moroccan Thyme was shown to be a source of numerous minerals which are necessary for health promotion. All antimicrobial testing, disc diffusion, MIC, and MBC tests revealed that thyme had potent antibacterial activity against all microorganisms tested. Staphylococcus aureus was the most susceptible bacterium, followed by Salmonella enterica and Escherichia coli. Additionally, thyme exhibited great antifungal efficacy against Candida albicans. The acute toxicity results indicated that the aqueous extract of T. vulgaris is almost non-toxic when taken orally. According to the chronic toxicity study, the extract is generally safe when taken orally over an extended period of time. The biochemical and haematological characteristics of the serum and blood were within acceptable limits, and histological examination revealed no abnormalities. In conclusion, the findings of this investigation, confirm the antimicrobial efficacy of the aqueous extract of Moroccan T. vulgaris and its safety for experimental animals.