茄科植物叶际原生生物中的Ciliophora和叶绿体优势

IF 3.3 3区 生物学 Q2 MICROBIOLOGY Phytobiomes Journal Pub Date : 2022-05-19 DOI:10.1094/pbiomes-04-22-0021-fi
Stephen J. Taerum, B. Steven, D. Gage, L. Triplett
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引用次数: 3

摘要

Phyllosphere微生物群在塑造植物健康和功能方面发挥着重要作用,但大多数研究只关注细菌和真菌群落。因此,人们对叶层中各种微孢子虫的组成知之甚少,包括原生捕食者和藻类,也不知道它们是如何受到宿主物种或共存细菌的影响的。使用通用真核引物和PNA夹具阻断植物18S rRNA基因的扩增,我们对美国康涅狄格州一块田地里生长的五种茄科作物的叶圈和根际微生物组进行了分析。Phyllosphere样本包含来自至少七个主要真核谱系的数百个原生生物序列变体,其中五分之一在大块土壤或根际样本中没有观察到。Phyllosphere样品高度富集了一些特定的序列变体,代表绿藻(绿藻目衣藻目)和纤毛虫(Ciliophora纲Colpodea),而根层则由Cercozoa门的原生生物主导。相关分析表明,鞘氨醇单胞菌是与叶际和根际原生生物呈正相关的中心网络枢纽。这些发现表明,虽然叶表面有一个多样性有限的高度可变的原生生物群落,但某些纤毛虫和绿藻可能很适合叶层栖息地。
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Dominance of Ciliophora and Chlorophyta among phyllosphere protists of Solanaceous plants
Phyllosphere microbiota play an important role in shaping plant health and function, but most research has focused only on bacterial and fungal communities. As a result, little is known about the composition of diverse microeukaryotes in the phyllosphere, including protist predators and algae, or how they are affected by host species or co-occurring bacteria. Using universal eukaryotic primers and a PNA clamp to block amplification of the plant 18S rRNA gene, we profiled the phyllosphere and rhizosphere microbiomes of five solanaceous crop species grown in a field plot in Connecticut, USA. Phyllosphere communities of protists, like those of bacteria, were far less diverse and more variable than those of the rhizosphere. Phyllosphere samples contained hundreds of protist sequence variants from at least seven major eukaryotic lineages, of which one-fifth were not observed in bulk soil or rhizosphere samples. Phyllosphere samples were highly enriched for a few specific sequence variants representing green algae (Chlorophyta order Chlamydomonales) and ciliates (Ciliophora class Colpodea), while rhizospheres were dominated by protists from the phylum Cercozoa. Correlation analysis identified Sphingomonas spp. bacteria as central network hubs positively associated with protists in the phyllosphere and rhizosphere. These findings indicate that while leaf surfaces host a highly variable protist community of limited diversity, certain ciliates and green algae may be well-adapted for the phyllosphere habitat.
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来源期刊
CiteScore
7.40
自引率
6.80%
发文量
42
审稿时长
4 weeks
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