红熊猫睾丸细胞分子标记的鉴定

IF 0.8 4区 生物学 Q3 ZOOLOGY Mammal Study Pub Date : 2021-06-02 DOI:10.3106/ms2020-0080
J. An, Ling He, R. Hou, Z. Cai, Dong-Hui Wang, Ke-Yu Shi, Songrui Liu, Chanjuan Yue, Yu-liang Liu
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引用次数: 0

摘要

摘要圈养大熊猫的自我维持种群受到繁殖成功率低的挑战。明确不同类型的睾丸细胞,增加对雄性生殖能力的了解,对于提高圈养大熊猫种群的生殖效率至关重要。本文首次报道了不同年龄大熊猫睾丸的组织学结构。此外,我们的数据还显示,红熊猫睾丸细胞的标志物,包括ITGA6、PLZF、THY1、VASA、DAZL、GATA4、VASA和αSMA,在成年睾丸组织中表达。为了进一步检测ITGA6、VASA、VIM和αSMA的定位,进行了免疫组织化学分析,结果分别显示了未分化精原细胞、多种生殖细胞、支持细胞和管周类肌细胞(PMCs)的典型染色模式。对于睾丸组织消化,使用两步酶消化来获得细胞悬浮液。本研究对表达支持细胞标志物VIM的大熊猫支持细胞进行了培养和保存,可用于建立大熊猫的支持细胞,也可作为大熊猫精原干细胞(SSCs)生长的饲养细胞。
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Characterization of Molecular Markers of Testicular Cells in Red Pandas (Ailurus fulgens styani)
Abstract. Self-sustained populations of captive red panda were challenged by low reproductive success rate. Defining the different types of testicular cell and increasing the knowledge of male fertility was critical for the improvement of reproductive efficiency in red panda captive populations. Here, we firstly report the histological structure in red panda testes at various ages. Besides, our data also showed that markers for red panda testicular cells including ITGA6, PLZF, THY1, VASA, DAZL, GATA4, VASA, and αSMA were expressed in adult testis tissue. To further examine the localizations of ITGA6, VASA, VIM, and αSMA, the immunohistochemical analysis was performed and the result showed the typical staining patterns of undifferentiated spermatogonia, multiple types of germ cells, Sertoli cells, and peritubular myoid cells (PMCs), respectively. For the testicular tissue digestion, two-step enzyme digestion was used to obtain the cell suspension. The red panda Sertoli cells, expressing Sertoli cell marker VIM, were cultured and conserved in this study, which can be used for red panda Sertoli cell establishment and as the feeder cells for red panda spermatogonial stem cells (SSCs) growing.
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来源期刊
Mammal Study
Mammal Study ZOOLOGY-
CiteScore
1.70
自引率
20.00%
发文量
23
审稿时长
>12 weeks
期刊介绍: Mammal Study is the official journal of the Mammal Society of Japan. It publishes original articles, short communications, and reviews on all aspects of mammalogy quarterly, written in English.
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