S. Carikci, T. Kilic, Züleyha Özer, T. Dirmenci, T. Arabaci, A. Gören
{"title":"杜鹃花中某些酚类物质的定量测定。经验证的LC-MS/MS法测定提取物的抗氧化活性","authors":"S. Carikci, T. Kilic, Züleyha Özer, T. Dirmenci, T. Arabaci, A. Gören","doi":"10.25135/JCM.21.18.11.1115","DOIUrl":null,"url":null,"abstract":"In this study, quantities of some phenolics; caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin in the directly methanol (M1), chloroform (C), acetone (Ac) and methanol (M2) extratcs obtained from Origanum laevigatum Boiss. from Turkey were investigated via liquid chromatography and tandem mass spectrometry (LC-MS/MS). Curcumin was used as an internal standard. Caffeic acid, chlorogenic acid, rosmarinic acid and gallic acid were determined as the most abundant phenolic acids in the studied extracts using LC-MS/MS. In LC-MS/MS measurements relative standard deviations (RSD %) for caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin were found to be 8.04, 5.21, 5.45, 3.73, 5.44, 4.85, 5.47 and 6.57 % respectively. For the investigated analytes, the correlation coefficient was found in the range of 0.9803 to 0.9981. Furthermore, antioxidant properties of the extracts were determined based on 2,2-diphenyl-1picrylhydrazyl (DPPH), β-carotene linoleic acid and cupric (Cu2) ion reducing power assay (CUPRAC). Acetone (Ac) and methanol (M2) extracts showed high activity in all test assays due to their high concentration of rosmarinic acid, caffeic acid and gallic acid in the extracts.","PeriodicalId":15343,"journal":{"name":"Journal of Chemical Metrology","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2018-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":"{\"title\":\"Quantitative determination of some phenolics in Origanum laevigatum Boiss. extracts via validated LC-MS/MS metod and antioxidant activity\",\"authors\":\"S. Carikci, T. Kilic, Züleyha Özer, T. Dirmenci, T. Arabaci, A. Gören\",\"doi\":\"10.25135/JCM.21.18.11.1115\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this study, quantities of some phenolics; caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin in the directly methanol (M1), chloroform (C), acetone (Ac) and methanol (M2) extratcs obtained from Origanum laevigatum Boiss. from Turkey were investigated via liquid chromatography and tandem mass spectrometry (LC-MS/MS). Curcumin was used as an internal standard. Caffeic acid, chlorogenic acid, rosmarinic acid and gallic acid were determined as the most abundant phenolic acids in the studied extracts using LC-MS/MS. In LC-MS/MS measurements relative standard deviations (RSD %) for caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin were found to be 8.04, 5.21, 5.45, 3.73, 5.44, 4.85, 5.47 and 6.57 % respectively. For the investigated analytes, the correlation coefficient was found in the range of 0.9803 to 0.9981. Furthermore, antioxidant properties of the extracts were determined based on 2,2-diphenyl-1picrylhydrazyl (DPPH), β-carotene linoleic acid and cupric (Cu2) ion reducing power assay (CUPRAC). Acetone (Ac) and methanol (M2) extracts showed high activity in all test assays due to their high concentration of rosmarinic acid, caffeic acid and gallic acid in the extracts.\",\"PeriodicalId\":15343,\"journal\":{\"name\":\"Journal of Chemical Metrology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2018-12-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Chemical Metrology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.25135/JCM.21.18.11.1115\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chemical Metrology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25135/JCM.21.18.11.1115","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Quantitative determination of some phenolics in Origanum laevigatum Boiss. extracts via validated LC-MS/MS metod and antioxidant activity
In this study, quantities of some phenolics; caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin in the directly methanol (M1), chloroform (C), acetone (Ac) and methanol (M2) extratcs obtained from Origanum laevigatum Boiss. from Turkey were investigated via liquid chromatography and tandem mass spectrometry (LC-MS/MS). Curcumin was used as an internal standard. Caffeic acid, chlorogenic acid, rosmarinic acid and gallic acid were determined as the most abundant phenolic acids in the studied extracts using LC-MS/MS. In LC-MS/MS measurements relative standard deviations (RSD %) for caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin were found to be 8.04, 5.21, 5.45, 3.73, 5.44, 4.85, 5.47 and 6.57 % respectively. For the investigated analytes, the correlation coefficient was found in the range of 0.9803 to 0.9981. Furthermore, antioxidant properties of the extracts were determined based on 2,2-diphenyl-1picrylhydrazyl (DPPH), β-carotene linoleic acid and cupric (Cu2) ion reducing power assay (CUPRAC). Acetone (Ac) and methanol (M2) extracts showed high activity in all test assays due to their high concentration of rosmarinic acid, caffeic acid and gallic acid in the extracts.