杜鹃花中某些酚类物质的定量测定。经验证的LC-MS/MS法测定提取物的抗氧化活性

IF 1.8 Q3 CHEMISTRY, ANALYTICAL Journal of Chemical Metrology Pub Date : 2018-12-22 DOI:10.25135/JCM.21.18.11.1115
S. Carikci, T. Kilic, Züleyha Özer, T. Dirmenci, T. Arabaci, A. Gören
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引用次数: 9

摘要

在这项研究中,一些酚类物质的数量;咖啡酸、(E)-阿威酸、迷迭香酸、绿原酸、富马酸、没食子酸、邻苯三酚和香兰素在直接甲醇(M1)、氯仿(C)、丙酮(Ac)和甲醇(M2)萃取物中的含量。采用液相色谱和串联质谱法(LC-MS/MS)对产自土耳其的黄芪多糖进行了研究。以姜黄素为内标。采用LC-MS/MS法测定了咖啡酸、绿原酸、迷迭香酸和没食子酸是提取物中含量最高的酚酸。咖啡酸、(E)-阿魏酸、迷迭香酸、绿原酸、富马酸、没食子酸、邻苯三酚和香兰素的相对标准偏差(RSD %)分别为8.04、5.21、5.45、3.73、5.44、4.85、5.47和6.57%。分析物的相关系数为0.9803 ~ 0.9981。以2,2-二苯基-1吡啶酰肼(DPPH)、β-胡萝卜素亚油酸和铜离子还原力(CUPRAC)测定提取物的抗氧化性能。丙酮(Ac)和甲醇(M2)提取物由于其含有高浓度的迷迭香酸、咖啡酸和没食子酸,在所有的试验中均表现出较高的活性。
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Quantitative determination of some phenolics in Origanum laevigatum Boiss. extracts via validated LC-MS/MS metod and antioxidant activity
In this study, quantities of some phenolics; caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin in the directly methanol (M1), chloroform (C), acetone (Ac) and methanol (M2) extratcs obtained from Origanum laevigatum Boiss. from Turkey were investigated via liquid chromatography and tandem mass spectrometry (LC-MS/MS). Curcumin was used as an internal standard. Caffeic acid, chlorogenic acid, rosmarinic acid and gallic acid were determined as the most abundant phenolic acids in the studied extracts using LC-MS/MS. In LC-MS/MS measurements relative standard deviations (RSD %) for caffeic acid, (E)-ferulic acid, rosmarinic acid, chlorogenic acid, fumaric acid, gallic acid, pyrogallol and vanillin were found to be 8.04, 5.21, 5.45, 3.73, 5.44, 4.85, 5.47 and 6.57 % respectively. For the investigated analytes, the correlation coefficient was found in the range of 0.9803 to 0.9981. Furthermore, antioxidant properties of the extracts were determined based on 2,2-diphenyl-1picrylhydrazyl (DPPH), β-carotene linoleic acid and cupric (Cu2) ion reducing power assay (CUPRAC). Acetone (Ac) and methanol (M2) extracts showed high activity in all test assays due to their high concentration of rosmarinic acid, caffeic acid and gallic acid in the extracts.
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来源期刊
Journal of Chemical Metrology
Journal of Chemical Metrology CHEMISTRY, ANALYTICAL-
CiteScore
2.30
自引率
15.40%
发文量
7
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