Jing Hu , Ming Zhao , Qimin Shi , Lingxin Li , Jian Yin
{"title":"基于配体的ELISA法检测人血清中可溶性asialal糖蛋白受体","authors":"Jing Hu , Ming Zhao , Qimin Shi , Lingxin Li , Jian Yin","doi":"10.1080/07328303.2021.2008952","DOIUrl":null,"url":null,"abstract":"Abstract A ligand-based enzyme-linked immunosorbent assay (ELISA) for the soluble form of functional asialoglycaoprotein receptor (s-ASGPR) in human serum has been developed. This assay was evaluated and proved to show good stability, which is suitable for testing human serum. Clinical serum samples were collected and tested with the established assay. The contents of serum s-ASGPR in healthy group and liver injury group were compared (p < 0.001). The functional s-ASGPR can be a promising candidate of noninvasive biomarker for liver function and injury. The receiver-operating characteristic (ROC) curve analysis illustrated that this economical, sensitive, and reproducible quantitative assay has great potential for clinical use. Graphical Abstract","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"40 7","pages":"Pages 440-453"},"PeriodicalIF":1.2000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A ligand-based ELISA for detection of soluble asialoglycoprotein receptor in human serum\",\"authors\":\"Jing Hu , Ming Zhao , Qimin Shi , Lingxin Li , Jian Yin\",\"doi\":\"10.1080/07328303.2021.2008952\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract A ligand-based enzyme-linked immunosorbent assay (ELISA) for the soluble form of functional asialoglycaoprotein receptor (s-ASGPR) in human serum has been developed. This assay was evaluated and proved to show good stability, which is suitable for testing human serum. Clinical serum samples were collected and tested with the established assay. The contents of serum s-ASGPR in healthy group and liver injury group were compared (p < 0.001). The functional s-ASGPR can be a promising candidate of noninvasive biomarker for liver function and injury. The receiver-operating characteristic (ROC) curve analysis illustrated that this economical, sensitive, and reproducible quantitative assay has great potential for clinical use. Graphical Abstract\",\"PeriodicalId\":15311,\"journal\":{\"name\":\"Journal of Carbohydrate Chemistry\",\"volume\":\"40 7\",\"pages\":\"Pages 440-453\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Carbohydrate Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/org/science/article/pii/S0732830322000155\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Carbohydrate Chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/org/science/article/pii/S0732830322000155","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
A ligand-based ELISA for detection of soluble asialoglycoprotein receptor in human serum
Abstract A ligand-based enzyme-linked immunosorbent assay (ELISA) for the soluble form of functional asialoglycaoprotein receptor (s-ASGPR) in human serum has been developed. This assay was evaluated and proved to show good stability, which is suitable for testing human serum. Clinical serum samples were collected and tested with the established assay. The contents of serum s-ASGPR in healthy group and liver injury group were compared (p < 0.001). The functional s-ASGPR can be a promising candidate of noninvasive biomarker for liver function and injury. The receiver-operating characteristic (ROC) curve analysis illustrated that this economical, sensitive, and reproducible quantitative assay has great potential for clinical use. Graphical Abstract
期刊介绍：
The Journal of Carbohydrate Chemistry serves as an international forum for research advances involving the chemistry and biology of carbohydrates. The following aspects are considered to fall within the scope of this journal:
-novel synthetic methods involving carbohydrates, oligosaccharides, and glycoconjugates-
the use of chemical methods to address aspects of glycobiology-
spectroscopic and crystallographic structure studies of carbohydrates-
computational and molecular modeling studies-
physicochemical studies involving carbohydrates and the chemistry and biochemistry of carbohydrate polymers.
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