2,4,4'-三羟基查尔酮的抗氧化活性及对肥胖相关消化酶的抑制作用

IF 0.3 4区农林科学 Q4 VETERINARY SCIENCES Slovenian Veterinary Research Pub Date : 2023-02-26 DOI:10.26873/svr-1625-2022

H. Alsufiani, Ghaynat A. Aldhaheri, U. Omar, Taraji M. Bahdilah, Rasha A Mansouri

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引用次数: 0

摘要

本研究旨在探讨2,4,4′-三羟基查尔酮对肥胖相关消化酶蔗糖酶、α-淀粉酶和脂肪酶的抗氧化活性和抑制作用。采用2,2-二苯基-1-苦味肼基自由基清除能力、还原能力和铁离子螯合能力测定3种浓度2,4,4'-三羟基查尔酮(100、300和500µg/ml)的体外抗氧化活性。此外，采用特异性测定法测定了2,4,4'-三羟基查尔酮对脂肪酶、α-淀粉酶和蔗糖酶活性的体外抑制作用。2,4,4'-三羟基查尔酮已被证明具有抗氧化性能，并抑制蔗糖酶、α-淀粉酶和脂肪酶的活性。这些结果表明2,4,4 ' -三羟基查尔酮具有抗氧化活性，可以有效抑制肥胖相关的关键酶。

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ANTIOXIDANT ACTIVITY AND INHIBITORY EFFECT OF 2,4,4'-TRIHYDROXYCHALCONE ON DIGESTIVE ENZYMES RELATED TO OBESITY

The aim of this study was to investigate the antioxidant activity and inhibitory effect of 2,4,4'-trihydroxychalcone on digestive enzymes related to obesity, including sucrase, α-amylase, and lipase. The in vitro antioxidant activities of three concentrations of 2,4,4'-trihydroxychalcone (100, 300, and 500 µg/ml) were determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging, reducing power, and ferrous ion chelating assays. Moreover, in vitro inhibition of lipase, α-amylase, and sucrase enzyme activities by 2,4,4'-trihydroxychalcone was determined using a specific assay for each enzyme. 2,4,4'-Trihydroxychalcone has been shown to have antioxidant properties and inhibits sucrase, α-amylase and lipase activities. These findings suggest that 2,4,4′-trihydroxychalcone demonstrated an antioxidant activity and can effectively inhibit the key enzymes related to obesity.

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来源期刊

Slovenian Veterinary Research VETERINARY SCIENCES-

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： SLOVENIAN VETERINARY RESEARCH (ISSN 1580-4003) publishes original articles, which report the results of original research in most areas of biomedicine. The journal also publishes review articles dealing with rapidly developing areas of biomedicine or which update understanding of classical fields of biomedicine, as well as case reports, shorter scientific contributions, letters to the editor, etc.; which have not been published or are under consideration for publication elsewhere. Only papers written in English can be considered.