{"title":"葡萄籽油对铬致大鼠肾毒性和氧化应激的改善作用","authors":"S. Orabi, S. Shawky","doi":"10.26873/svr-967-2020","DOIUrl":null,"url":null,"abstract":"The current study focused on investigating the renoprotective effects of grape seed oil (GSO) against hexavalent chromium (Cr (VI))-induced nephrotoxicity. A total of 40 male rats were randomly divided into four groups: group I served as the control group, group II received 1000 mg/L potassium dichromate (353.5 mg/L Cr(VI)) in drinking water for 12 weeks, group III received 3.7 g/kg body weight/day GSO orally for 12 weeks, and group IV received GSO together with potassium dichromate for 12 weeks. Cr(VI) significantly increased serum levels of urea, creatinine, potassium and glucose. In addition, Cr(VI) increased MDA levels and induced renal tissue damage and DNA damage. On the other hand, Cr(VI) decreased serum levels of sodium and antioxidant defence system [reduced glutathione (GSH) and catalase (CAT)]. However, treatment with GSO prevented elevation levels of serum urea, creatinine, potassium and glucose. In addition, GSO enhanced sodium level, renal tissue antioxidant defense system due to its curative effect ameliorated particularly oxidative stress, renal tissue and DNA damage. In conclusion, these results demonstrate that GSO is a promising nephroprotective agent against Cr(VI)-induced nephrotoxicity. Key words: grape seed oil; hexavalent chromium; nephrotoxicity; DNA damage BLAŽILNI UCINKI OLJA GROZDNIH PESK PRI TOKSICNI OBREMENITVI LEDVIC TER VPLIV NA OKSIDATIVNI STRES PODGAN, POVZROCEN S KROMOM Povzetek: Studija je bila osredotocena na proucevanje zascitnih ucinkov olja grozdnih pesk (GSO) pri toksicni obremenitvi ledvic, povzroceni s heksavalentnim kromom (Cr (VI)). Stirideset samcev podgan je bilo nakljucno razdeljenih v stiri skupine: skupina I - kontrolna skupina, skupina II, ki je v pitni vodi 12 tednov prejemala 1000 mg/L kalijevega dikromata (353,5 mg/L Cr (VI)), skupina III, ki je peroralno 12 tednov prejemala 3,7 g/kg telesne mase/dan GSO ter skupina IV, ki je 12 tednov prejemala GSO skupaj s kalijevim dikromatom. Cr(VI) je znatno zvisal serumske ravni secnine, kreatinina, kalija in glukoze v serumu. Poleg tega je Cr(VI) zvisal raven MDA in povzrocil poskodbe ledvicnega tkiva in poskodbe DNK. Po drugi strani je Cr(VI) znižal serumsko raven natrija in antioksidativnega obrambnega sistema, zmanjsal raven glutationske peroksidaze in katalaze. Dodajanje GSO poskusnim živalim je preprecilo zvisanje ravni secnine v serumu, kreatinina, kalija, natrija in glukoze. Poleg tega je GSO izboljsal obrambni sistem antioksidantov ledvicnega tkiva. Zaradi svojega zdravilnega ucinka je izboljsal zlasti oksidativni stres, poskodbe ledvicnega tkiva in DNK. Rezultati kažejo, da je GSO obetavno zascitno sredstvo za ledvica pri toksicni obremenitvi, povzroceni s Cr(VI). Kljucne besede: olje grozdnih pesk; heksavalentni krom; nefrotoksicnost; poskodba DNK","PeriodicalId":21765,"journal":{"name":"Slovenian Veterinary Research","volume":" ","pages":""},"PeriodicalIF":0.3000,"publicationDate":"2020-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"AMELIORATIVE EFFECTS OF GRAPE SEED OIL ON CHROMIUM-INDUCED NEPHROTOXICITY AND OXIDATIVESTRESS IN RATS\",\"authors\":\"S. Orabi, S. Shawky\",\"doi\":\"10.26873/svr-967-2020\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The current study focused on investigating the renoprotective effects of grape seed oil (GSO) against hexavalent chromium (Cr (VI))-induced nephrotoxicity. A total of 40 male rats were randomly divided into four groups: group I served as the control group, group II received 1000 mg/L potassium dichromate (353.5 mg/L Cr(VI)) in drinking water for 12 weeks, group III received 3.7 g/kg body weight/day GSO orally for 12 weeks, and group IV received GSO together with potassium dichromate for 12 weeks. Cr(VI) significantly increased serum levels of urea, creatinine, potassium and glucose. In addition, Cr(VI) increased MDA levels and induced renal tissue damage and DNA damage. On the other hand, Cr(VI) decreased serum levels of sodium and antioxidant defence system [reduced glutathione (GSH) and catalase (CAT)]. However, treatment with GSO prevented elevation levels of serum urea, creatinine, potassium and glucose. In addition, GSO enhanced sodium level, renal tissue antioxidant defense system due to its curative effect ameliorated particularly oxidative stress, renal tissue and DNA damage. In conclusion, these results demonstrate that GSO is a promising nephroprotective agent against Cr(VI)-induced nephrotoxicity. Key words: grape seed oil; hexavalent chromium; nephrotoxicity; DNA damage BLAŽILNI UCINKI OLJA GROZDNIH PESK PRI TOKSICNI OBREMENITVI LEDVIC TER VPLIV NA OKSIDATIVNI STRES PODGAN, POVZROCEN S KROMOM Povzetek: Studija je bila osredotocena na proucevanje zascitnih ucinkov olja grozdnih pesk (GSO) pri toksicni obremenitvi ledvic, povzroceni s heksavalentnim kromom (Cr (VI)). Stirideset samcev podgan je bilo nakljucno razdeljenih v stiri skupine: skupina I - kontrolna skupina, skupina II, ki je v pitni vodi 12 tednov prejemala 1000 mg/L kalijevega dikromata (353,5 mg/L Cr (VI)), skupina III, ki je peroralno 12 tednov prejemala 3,7 g/kg telesne mase/dan GSO ter skupina IV, ki je 12 tednov prejemala GSO skupaj s kalijevim dikromatom. Cr(VI) je znatno zvisal serumske ravni secnine, kreatinina, kalija in glukoze v serumu. Poleg tega je Cr(VI) zvisal raven MDA in povzrocil poskodbe ledvicnega tkiva in poskodbe DNK. Po drugi strani je Cr(VI) znižal serumsko raven natrija in antioksidativnega obrambnega sistema, zmanjsal raven glutationske peroksidaze in katalaze. Dodajanje GSO poskusnim živalim je preprecilo zvisanje ravni secnine v serumu, kreatinina, kalija, natrija in glukoze. Poleg tega je GSO izboljsal obrambni sistem antioksidantov ledvicnega tkiva. Zaradi svojega zdravilnega ucinka je izboljsal zlasti oksidativni stres, poskodbe ledvicnega tkiva in DNK. Rezultati kažejo, da je GSO obetavno zascitno sredstvo za ledvica pri toksicni obremenitvi, povzroceni s Cr(VI). Kljucne besede: olje grozdnih pesk; heksavalentni krom; nefrotoksicnost; poskodba DNK\",\"PeriodicalId\":21765,\"journal\":{\"name\":\"Slovenian Veterinary Research\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.3000,\"publicationDate\":\"2020-10-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Slovenian Veterinary Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.26873/svr-967-2020\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Slovenian Veterinary Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.26873/svr-967-2020","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 4
摘要
本研究主要探讨葡萄籽油(GSO)对六价铬(Cr (VI))所致肾毒性的保护作用。选取40只雄性大鼠，随机分为4组:ⅰ组为对照组，ⅱ组为饮用水中1000mg /L重铬酸钾(353.5 mg/L Cr(VI))，连续12周，ⅲ组为3.7 g/kg体重/天口服GSO，连续12周，ⅳ组为GSO与重铬酸钾联合用药，连续12周。铬(VI)显著提高血清尿素、肌酐、钾和葡萄糖水平。此外，Cr(VI)增加MDA水平，引起肾组织损伤和DNA损伤。另一方面，Cr(VI)降低了血清钠和抗氧化防御系统[还原性谷胱甘肽(GSH)和过氧化氢酶(CAT)]的水平。然而，用GSO治疗可防止血清尿素、肌酐、钾和葡萄糖水平升高。此外，GSO还能提高钠水平，改善肾组织抗氧化防御系统，特别是改善氧化应激、肾组织和DNA损伤。综上所述，GSO是一种很有前途的抗Cr(VI)肾毒性的肾保护剂。关键词:葡萄籽油;六价铬;肾毒性;DNA损伤BLAŽILNI UCINKI OLJA GROZDNIH PESK PRI TOKSICNI OBREMENITVI LEDVIC TER VPLIV NA OKSIDATIVNI STRES PODGAN, Povzetek: Studija bila osredotocena NA procevanje zascitnih ucinkov OLJA GROZDNIH PESK (GSO) PRI TOKSICNI OBREMENITVI LEDVIC, povzroceni S heksavalentnim KROMOM (Cr (VI)))。Stirideset samcev podgan je bilo nakljucno razdeljenih v stiri skupine: skupina I - control skupina, skupina II, ki jev pitni vodi 12 tetenov prejemala 1000 mg/L kalijevega dikromata (353,5 mg/L Cr (VI))， skupina III, ki je peroralno 12 tetenov prejemala 3,7 g/kg telesonase /dan GSO ter skupina IV, ki je 12 tetenov prejemala GSO skupajs kalijevim dikrooma。Cr(VI) je znatno zvisualser血清，ravni secine, creatiina, kalija在葡萄糖和血清中。Poleg tega je Cr(VI) zvisualraven MDA在povzrocil可能被ledvicnega tkiva在可能的DNK。Po药物strani je Cr(VI) znižal血清中raven natrija抗氧化性脑炎系统，zmanjsal raven glutglutaske peroksidaze在katalaze。Dodajanje GSO poskusnim živalim je preprecilo zvisanje ravni secnine v血清，kreatiina, kalija, natrija在胶合。Poleg tega je GSO izboljsal obramni系统抗氧化性研究进展。Zaradi svojega zdravilnega ucinka je izboljsal zlasti oksitiveni stress，可能是DNK的dna。Rezultati kažejo, da je GSO obetavno zascitno sredstvo zbetavno zascitno sredstvo zledvica ptoksicni remremiti, povzroceni s Cr(VI)。kljune beede: olje grozdnih pesk;heksavalentni krom;nefrotoksicnost;poskodba DNK
AMELIORATIVE EFFECTS OF GRAPE SEED OIL ON CHROMIUM-INDUCED NEPHROTOXICITY AND OXIDATIVESTRESS IN RATS
The current study focused on investigating the renoprotective effects of grape seed oil (GSO) against hexavalent chromium (Cr (VI))-induced nephrotoxicity. A total of 40 male rats were randomly divided into four groups: group I served as the control group, group II received 1000 mg/L potassium dichromate (353.5 mg/L Cr(VI)) in drinking water for 12 weeks, group III received 3.7 g/kg body weight/day GSO orally for 12 weeks, and group IV received GSO together with potassium dichromate for 12 weeks. Cr(VI) significantly increased serum levels of urea, creatinine, potassium and glucose. In addition, Cr(VI) increased MDA levels and induced renal tissue damage and DNA damage. On the other hand, Cr(VI) decreased serum levels of sodium and antioxidant defence system [reduced glutathione (GSH) and catalase (CAT)]. However, treatment with GSO prevented elevation levels of serum urea, creatinine, potassium and glucose. In addition, GSO enhanced sodium level, renal tissue antioxidant defense system due to its curative effect ameliorated particularly oxidative stress, renal tissue and DNA damage. In conclusion, these results demonstrate that GSO is a promising nephroprotective agent against Cr(VI)-induced nephrotoxicity. Key words: grape seed oil; hexavalent chromium; nephrotoxicity; DNA damage BLAŽILNI UCINKI OLJA GROZDNIH PESK PRI TOKSICNI OBREMENITVI LEDVIC TER VPLIV NA OKSIDATIVNI STRES PODGAN, POVZROCEN S KROMOM Povzetek: Studija je bila osredotocena na proucevanje zascitnih ucinkov olja grozdnih pesk (GSO) pri toksicni obremenitvi ledvic, povzroceni s heksavalentnim kromom (Cr (VI)). Stirideset samcev podgan je bilo nakljucno razdeljenih v stiri skupine: skupina I - kontrolna skupina, skupina II, ki je v pitni vodi 12 tednov prejemala 1000 mg/L kalijevega dikromata (353,5 mg/L Cr (VI)), skupina III, ki je peroralno 12 tednov prejemala 3,7 g/kg telesne mase/dan GSO ter skupina IV, ki je 12 tednov prejemala GSO skupaj s kalijevim dikromatom. Cr(VI) je znatno zvisal serumske ravni secnine, kreatinina, kalija in glukoze v serumu. Poleg tega je Cr(VI) zvisal raven MDA in povzrocil poskodbe ledvicnega tkiva in poskodbe DNK. Po drugi strani je Cr(VI) znižal serumsko raven natrija in antioksidativnega obrambnega sistema, zmanjsal raven glutationske peroksidaze in katalaze. Dodajanje GSO poskusnim živalim je preprecilo zvisanje ravni secnine v serumu, kreatinina, kalija, natrija in glukoze. Poleg tega je GSO izboljsal obrambni sistem antioksidantov ledvicnega tkiva. Zaradi svojega zdravilnega ucinka je izboljsal zlasti oksidativni stres, poskodbe ledvicnega tkiva in DNK. Rezultati kažejo, da je GSO obetavno zascitno sredstvo za ledvica pri toksicni obremenitvi, povzroceni s Cr(VI). Kljucne besede: olje grozdnih pesk; heksavalentni krom; nefrotoksicnost; poskodba DNK
期刊介绍：
SLOVENIAN VETERINARY RESEARCH (ISSN 1580-4003) publishes original articles, which report the results of original research in most areas of biomedicine. The journal also publishes review articles dealing with rapidly developing areas of biomedicine or which update understanding of classical fields of biomedicine, as well as case reports, shorter scientific contributions, letters to the editor, etc.; which have not been published or are under consideration for publication elsewhere. Only papers written in English can be considered.
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