土耳其家猫细小病毒VP2部分与全基因序列的比较

IF 0.2 4区 农林科学 Q2 Veterinary Veterinaria Mexico Pub Date : 2019-12-11 DOI:10.22201/fmvz.24486760e.2019.4.643
Z. Akkutay-Yoldar, B. Taylan Koç
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引用次数: 2

摘要

墨西哥兽医OA ISSN:2448-6760引用为:Akkutay Yoldar Z,Taylan Koc B.土耳其家猫细小病毒VP2部分和几乎全部基因序列的分子特征。兽医墨西哥OA。2019年;6(4)。doi:10.22201/fmvz.24486760e.2019.4.643.细小病毒是一种普遍存在的病原体,会在猫身上引起致命疾病,并能够变异以进行跨物种传播。猫全白细胞减少症病毒(FPV)和犬细小病毒(CPV)及其抗原变体都会在猫身上引发一种症状相似的疾病。本研究的目的是确定五只临床症状猫(来自土耳其安卡拉)的血液和渗出液样本中是否存在细小病毒。通过聚合酶链式反应(PCR)扩增编码所获得的细小病毒的VP2结构衣壳蛋白的基因,纯化并部分或几乎全长测序。最大似然(ML)法用于分子表征。基于VP2基因和氨基酸排列的近全长测序的系统发育分析表明,四种病毒株密切相关,并定位在同一FPV簇中。发现的第五个菌株位于同一个集群中,但在一个单独的分支上。通过部分基因组分析确定,病毒场菌株被纳入CPV-2组:四株符合CPV-2c,一株在CPV-2b组内的单独分支中。据我们所知,这是第一份详细描述土耳其猫近全长VP2基因特征的报告。总的来说,与部分长度的比较相比,几乎全长的VP2对比更有效地确定细小病毒株的起源。
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Comparison of partial and full VP2 gene sequences of parvovirus from domestic cats in Turkey
Veterinaria Mexico OA ISSN: 2448-6760Cite this as:Akkutay-Yoldar Z, Taylan-Koc B. Molecular characterization of partial and nearly full parvovirus VP2 gene sequences from Turkish domestic cats. Veterinaria Mexico OA. 2019;6(4). doi: 10.22201/fmvz.24486760e.2019.4.643..Parvoviruses are ubiquitous pathogens that cause a fatal disease in cats and are able to mutate for cross-species transmission. Both the feline panleukopenia virus (FPV) and the canine parvovirus (CPV), with their antigenic variants, induce a disease in cats that presents with similar signs. The aim of this study was to determine the presence of parvoviruses in blood and exudate samples from five clinically symptomatic cats (from Ankara, Turkey). The gene coding for the VP2 structural capsid protein of the obtained parvoviruses was amplified by polymerase chain reaction (PCR), purified and partially or nearly full-length sequenced. The maximum likelihood (ML) method was used for molecular characterization. Phylogenetic analysis based on nearly full-length sequencing of the VP2 gene and amino acid arrangement showed that four of the viral strains were closely related and localized in the same FPV cluster. The fifth strain found was located in the same cluster but on a separate branch. Viral field strains were included in the CPV-2 group as determined by partial genome analysis: four fitted in the CPV-2c, and one in a separate clade within the CPV-2b group. To our knowledge, this is the first report that details nearly full-length VP2 gene characterisation in Turkish cats. Overall, nearly full-length VP2 contrasts were more effective to determine the origin of parvovirus strains, than partial length comparisons.
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来源期刊
Veterinaria Mexico
Veterinaria Mexico VETERINARY SCIENCES-
自引率
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审稿时长
>12 weeks
期刊介绍: Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).
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