在摩尔多瓦共和国苹果脱毒种植材料生产系统中的应用方法

C. Dadu, L. Prodanyuk, I. Kalashyan
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引用次数: 0

摘要

研究了苹果砧木mm106离体微繁技术。吲哚-3-丁酸(IBA)浓度为1 ~ 2 mg/l的变异生根率为80% ~ 100%。获得了苹果茎凹陷病毒(ASPV)诊断性多克隆抗血清。介绍了采用热疗法对苹果品种‘Coreor’、‘Coredova’、‘Coretard’、‘Izvoras’进行灭毒试验的结果。为了增加和保存梨果类作物的脱毒基因,在研究所的果圃中种植了脱毒无性系类前基。
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Methods applied in the production system of virus free planting material of apple in the Republic of Moldova
The technology of in vitro micropropagation of the apple rootstock MM 106 was developed. From 80 to100% rooting was obtained in variants with indole-3-butyric acid (IBA) 1-2 mg/l. A diagnostic polyclonal antiserum to the apple stem pitting virus (ASPV) was obtained. The results of the virus elimination of apple cultivars 'Coreor', 'Coredova', 'Coretard', 'Izvoras' genetically resistant to scab Venturia inaequalis Cooke Wint using methods of thermotherapy are described. The virus free clone’s category Prebase were planted in the depozitarium of the institute in order to increase and preserve the virus-free genefond of pome fruit crops.
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