浸渍乙醇和天然深共晶溶剂微波辅助提取桑叶提取物的优化研究

Aan Kunaedi, I. Indawati, N. Karlina, Zakie Fadhillah, Citra Dhea Cantika, Ade Ayu Khulfiah
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引用次数: 0

摘要

桑叶的乙醇提取物,一种草本植物,已被广泛用于医药,包括商业配方。本研究的目的是比较MAE法和浸渍法提取的NADES提取物的特性。桑叶(桑叶)L)乙醇提取物分别以不同浓度的乙醇(50%、60%、70%、80%、90%)和溶剂比为1:20、1:30的NADES(天然深共晶溶剂)提取物,微波辅助萃取(MAE)法提取,时间分别为5、10分钟。以槲皮素和没食子酸为参比标准品,采用紫外-可见分光光度法测定其总黄酮和总酚含量。结果表明:乙醇浓度为50%、60%、70%、80%和90%时,总黄酮含量平均;总酚:0.73%、0.9%、1.32%、1.18%、0.77%;总酚96.789 mg/g、71.262 mg/g、126.465 mg/g、116.643 mg/g、93.366 mg/g。NADES提取物在MAE 5"和10" min时,溶剂比为1:20,黄酮类化合物含量分别为0.36%、0.4%,总酚含量为36.099 mg/g;75.621毫克/克。以1:30的比例,浸泡时间分别为5"和10" min,黄酮类化合物含量为0.44%;0.48%,总酚含量为61.884 mg/g;121.237毫克/克。乙醇提取物水溶性精华液测定结果;NADES为26%-31%;21 - 24%。乙醇浸出物含量测定结果;NADES在36% ~ 42%之间;85% - -86%。乙醇萃取物比重结果;NADES范围为0.82 g/mL ~ 0.95 g/mL;1.08 g/mL - 1.18 g/mL。乙醇提取物pH值试验结果为;NADES在6.90-7.01之间;7.59 - -7.89。
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STUDY ON THE OPTIMIZATION OF MULBERRY LEAF EXTRACT BY MACERATING ETHANOL AND MICROWAVE ASSISTED EXTRACTION METHOD (MAE) WITH NATURAL DEEP EUTECTIC SOLVENTS (NADES)
Ethanol extract from the leaves of Morus alba, an herb, has been widely used for medicine, including commercial formulations. This study aims to compare the characteristics of NADES extract using the MAE method and the maceration method. Mulberry leaf simplicia (Morus alba. L) ethanol extract was made with various ethanol concentrations of 50%, 60%, 70%, 80%, and 90%, and NADES (Natural Deep Eutectic Solvents) extract with a solvent ratio of 1:20 and 1: 30 on the MAE (Microwave Assisted Extraction) method with a time of 5 and 10 minutes. Measurement of total flavonoid content and total phenolic content was carried out by UV-vis spectrophotometry with quercetin and gallic acid as reference standards. The results showed that the ethanol extract with ethanol concentrations of 50%, 60%, 70%, 80%, and 90% had an average total flavonoid content; and total phenolic: 0.73%, 0.9%, 1.32%, 1.18%, 0.77%; total phenolic 96.789 mg/g, 71.262 mg/g, 126.465 mg/g, 116.643 mg/g, 93.366 mg/g. NADES extract with a solvent ratio of 1:20 at MAE 5" and 10" minutes obtained flavonoid levels of 0.36%, 0.4%, and total phenolic levels of 36.099 mg/g; 75.621 mg/g. While a ratio of 1:30 with an MAE time of 5" and 10" minutes, the flavonoid content was 0.44%; 0.48%, and the total phenolic content of 61.884 mg/g; 121.237 mg/g. Results of water-soluble essence of ethanol extract; NADES is 26%–31%; 21–24%. Result of ethanol soluble extract content of ethanol extract; NADES is in the range of 36%–42%; 85%–86%. Result of the specific gravity of ethanol extract; NADES is in the range of 0.82 g/mL–0.95 g/mL; 1.08 g/mL–1.18 g/mL. The ethanol extract pH test results were; NADES in the range 6.90–7.01; 7.59–7.89.
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