Colorimetric estimation of vancomycin using modified silver nanoparticles and implementing it on various types of pharmaceutical perpetrations

Abstract. Al-Karami MA, Al-Rufaie MM. 2022. Colorimetric estimation of vancomycin using modified silver nanoparticles and implementing it on various types of pharmaceutical perpetrations. Nusantara Bioscience 14: 70-77. The current research proposes a simple, quick, and sensitive method for determining the pure state of vancomycin (Van) and several of its medicinal formulations. The antibiotic (Van) is an effective reduction agent for diluting the ore mineral salt from silver nitrate (Ag+) to silver nanoparticles. Polyvinylpyrrolidone was used as a stabilizer in a medium using sodium hydroxide as a base. The highest absorption of the vancomycin oxidation-reduction product occurs at 440 nm. The calibration curve is measured, and the following data is generated, indicating that the Beer-Lambert Law is followed within the concentration range of 2.5-70 parts per million. Sandal's molar absorptivity was 2.0043 x 104 L/mol.cm, and its sensitivity was 0.0723 g/cm2. The correlation coefficient was 0.9987, and the standard deviation rate was 0.402%. The biological influence of several Gram-positive and Gram-negative bacteria was researched, and the efficacy of the samples prepared on these bacteria has shown to be promising.