Preventive Supplementation of Vitamin E and Selenium as a Factor in Improving the Success Rate of Embryo Transfer in Cattle

Abstract The effects of stress on processes in the body are becoming an increasingly relevant research subject. The reproductive ability of bovine animals largely depends on these effects, whilst embryo transfer is increasingly being used as a reproduction method. In this study, we established the differences in the implantation ability of heifers that were treated (N=17) with selenium (Se) and vitamins AD3E, and non-treated heifers. Upon transfer, we took blood samples from both groups and used the total antioxidant status (TAS) value to analyze the presence of reactive oxygen species (ROS), the levels of non-esterified fatty acids (NEFA) and the levels of vitamin E and Se in blood plasma. In the study, we were able to demonstrate that preventive measures in the form of supplementation of vitamin E and Se, mitigate the effects of oxidative stress, strengthen the ability of an organism to improve the dynamic relationship between free radicals and antioxidants, improve the energy status of cattle, positively impact reproductive parameters and increase the success rate of embryo transfer. The difference in the number of successful embryo implantations between the control and treated group was statistically significant, with 64.7% of treated heifers being pregnant after embryo transfer and giving birth to healthy calves. In the control group, the implantation success rate was 41.2%. The supplementation of antioxidants in the form of a combination of vitamin AD3E and Se, proved to be a good method for strengthening the defense of an organism and an effective mean of preventive clinical approach for improving fertility parameters.