COUP-TFI缺失影响小鼠胎盘血管生成和凋亡相关基因表达的探索性研究结果

IF 0.4 4区 医学 Q4 OBSTETRICS & GYNECOLOGY Clinical and experimental obstetrics & gynecology Pub Date : 2022-01-10 DOI:10.31083/j.ceog4901007
L. Viola, S. Marzinotto, M. Bertacchi, A. Londero, M. Orsaria, S. Bertozzi, L. Driul, C. di Loreto, M. Studer, L. Mariuzzi, A. Fruscalzo
{"title":"COUP-TFI缺失影响小鼠胎盘血管生成和凋亡相关基因表达的探索性研究结果","authors":"L. Viola, S. Marzinotto, M. Bertacchi, A. Londero, M. Orsaria, S. Bertozzi, L. Driul, C. di Loreto, M. Studer, L. Mariuzzi, A. Fruscalzo","doi":"10.31083/j.ceog4901007","DOIUrl":null,"url":null,"abstract":"Background: Chicken Ovalbumin Upstream Promoter-Transcription Factor I (COUP-TFI) is a member of the steroid/thyroid nuclear receptor superfamily. The aim of this study was to investigate whether absence of this gene affects placental development and fetal growth in a COUP-TFI knockout mouse model. Methods: Placentas of COUP-TFI-knockout (COUP-TFI KO) and wild-type (WT) were collected at 18.5 days post-coitum. The expression level of the following genes known to be involved in different key molecular pathways was evaluated: BCL2Associated X (Bax) and B-cell lymphoma 2 (Bcl-2) (apoptosis), p21, p53 andα subunit of inhibin (INHA) (proliferation and apoptosis), vascular endothelial growth factor A (VEGF-A), placental growth factor (PlGF), hypoxia-inducible factor 1-alpha (HIF1α), Fms related receptor tyrosine kinase 1 (Flt-1), and endoglin (ENG) (angiogenesis). Mouse litter weight at birth was also assessed. Results: RT-qPCR analysis showed increased mRNA expression of VEGF-A and Bax in placental tissue of COUP-TFI KO mice compared to WT mice. We also found a loss in the positive correlation between Bcl-2 and INHA, p21 and ENG, as well as HIF1α and Flt-1 mRNA expression in COUP-TFI mutants. Finally, KO mice were lighter than WT littermates (respectively, the mean weight of COUP-TFI KOmice was 1.3 grams,± 0.13, compared to 1.6 g,± 0.14 ofWTmice, p< 0.05). Conclusions: Our results show that COUP-TFI deletion is associated with a lower birth weight in mice and increased placental transcript expression of pro-apoptotic Bax and pro-angiogenetic VEGF-A genes.","PeriodicalId":10312,"journal":{"name":"Clinical and experimental obstetrics & gynecology","volume":" ","pages":""},"PeriodicalIF":0.4000,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"COUP-TFI deletion affects angiogenesis and apoptosis related gene expression in mouse placenta: results of an explorative study\",\"authors\":\"L. Viola, S. Marzinotto, M. Bertacchi, A. Londero, M. Orsaria, S. Bertozzi, L. Driul, C. di Loreto, M. Studer, L. Mariuzzi, A. Fruscalzo\",\"doi\":\"10.31083/j.ceog4901007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Chicken Ovalbumin Upstream Promoter-Transcription Factor I (COUP-TFI) is a member of the steroid/thyroid nuclear receptor superfamily. The aim of this study was to investigate whether absence of this gene affects placental development and fetal growth in a COUP-TFI knockout mouse model. Methods: Placentas of COUP-TFI-knockout (COUP-TFI KO) and wild-type (WT) were collected at 18.5 days post-coitum. The expression level of the following genes known to be involved in different key molecular pathways was evaluated: BCL2Associated X (Bax) and B-cell lymphoma 2 (Bcl-2) (apoptosis), p21, p53 andα subunit of inhibin (INHA) (proliferation and apoptosis), vascular endothelial growth factor A (VEGF-A), placental growth factor (PlGF), hypoxia-inducible factor 1-alpha (HIF1α), Fms related receptor tyrosine kinase 1 (Flt-1), and endoglin (ENG) (angiogenesis). Mouse litter weight at birth was also assessed. Results: RT-qPCR analysis showed increased mRNA expression of VEGF-A and Bax in placental tissue of COUP-TFI KO mice compared to WT mice. We also found a loss in the positive correlation between Bcl-2 and INHA, p21 and ENG, as well as HIF1α and Flt-1 mRNA expression in COUP-TFI mutants. Finally, KO mice were lighter than WT littermates (respectively, the mean weight of COUP-TFI KOmice was 1.3 grams,± 0.13, compared to 1.6 g,± 0.14 ofWTmice, p< 0.05). Conclusions: Our results show that COUP-TFI deletion is associated with a lower birth weight in mice and increased placental transcript expression of pro-apoptotic Bax and pro-angiogenetic VEGF-A genes.\",\"PeriodicalId\":10312,\"journal\":{\"name\":\"Clinical and experimental obstetrics & gynecology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2022-01-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical and experimental obstetrics & gynecology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.31083/j.ceog4901007\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"OBSTETRICS & GYNECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and experimental obstetrics & gynecology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.31083/j.ceog4901007","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 2

摘要

背景:鸡卵清蛋白上游启动子转录因子I(COUP-TFI)是类固醇/甲状腺核受体超家族的成员。本研究的目的是研究该基因的缺失是否会影响COUP-TFI敲除小鼠模型中的胎盘发育和胎儿生长。方法:在交配后18.5天采集COUP-TFI敲除(COUP-TFI-KO)和野生型(WT)胎盘。评估了已知参与不同关键分子途径的以下基因的表达水平:BCL2相关X(Bax)和B细胞淋巴瘤2(Bcl-2)(凋亡)、p21、p53和抑制素α亚基(INHA)(增殖和凋亡)、血管内皮生长因子A(VEGF-A)、胎盘生长因子(PlGF)、缺氧诱导因子1α,Fms相关受体酪氨酸激酶1(Flt-1)和endoglin(ENG)(血管生成)。还对出生时的小鼠产仔重量进行了评估。结果:RT-qPCR分析显示,与WT小鼠相比,COUP-TFI KO小鼠胎盘组织中VEGF-A和Bax的mRNA表达增加。我们还发现,在COUP-TFI突变体中,Bcl-2与INHA、p21与ENG以及HIF1α和Flt-1mRNA表达之间的正相关性缺失。最后,KO小鼠比同窝出生的WT小鼠轻(COUP-TFI KO小鼠的平均体重分别为1.3克,±0.13,而WT小鼠的平均重量为1.6克,±0.14,p<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
COUP-TFI deletion affects angiogenesis and apoptosis related gene expression in mouse placenta: results of an explorative study
Background: Chicken Ovalbumin Upstream Promoter-Transcription Factor I (COUP-TFI) is a member of the steroid/thyroid nuclear receptor superfamily. The aim of this study was to investigate whether absence of this gene affects placental development and fetal growth in a COUP-TFI knockout mouse model. Methods: Placentas of COUP-TFI-knockout (COUP-TFI KO) and wild-type (WT) were collected at 18.5 days post-coitum. The expression level of the following genes known to be involved in different key molecular pathways was evaluated: BCL2Associated X (Bax) and B-cell lymphoma 2 (Bcl-2) (apoptosis), p21, p53 andα subunit of inhibin (INHA) (proliferation and apoptosis), vascular endothelial growth factor A (VEGF-A), placental growth factor (PlGF), hypoxia-inducible factor 1-alpha (HIF1α), Fms related receptor tyrosine kinase 1 (Flt-1), and endoglin (ENG) (angiogenesis). Mouse litter weight at birth was also assessed. Results: RT-qPCR analysis showed increased mRNA expression of VEGF-A and Bax in placental tissue of COUP-TFI KO mice compared to WT mice. We also found a loss in the positive correlation between Bcl-2 and INHA, p21 and ENG, as well as HIF1α and Flt-1 mRNA expression in COUP-TFI mutants. Finally, KO mice were lighter than WT littermates (respectively, the mean weight of COUP-TFI KOmice was 1.3 grams,± 0.13, compared to 1.6 g,± 0.14 ofWTmice, p< 0.05). Conclusions: Our results show that COUP-TFI deletion is associated with a lower birth weight in mice and increased placental transcript expression of pro-apoptotic Bax and pro-angiogenetic VEGF-A genes.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
0.50
自引率
0.00%
发文量
241
审稿时长
1 months
期刊介绍: CEOG is an international, peer-reviewed, open access journal. CEOG covers all aspects of Obstetrics and Gynecology, including obstetrics, prenatal diagnosis, maternal-fetal medicine, perinatology, general gynecology, gynecologic oncology, uro-gynecology, reproductive medicine, infertility, reproductive endocrinology, sexual medicine. All submissions of cutting-edge advances of medical research in the area of women''s health worldwide are encouraged.
期刊最新文献
Maternal Deaths in Kayseri: Causes and Risk Factors The Relationship between Nifedipine and Postpartum Blood Loss in Patients with Preterm Labor Add-Back and Combined Regulation in GnRH-a Treatment of Endometriosis The Transvaginal Natural Orifice Transluminal Endoscopic Surgery (vNOTES) Procedures in Contemporary Gynecology: An Appraisal of the Published Evidence and a Review A Retrospective Study Comparing of Group B Streptococcus Invasiveness in Pregnant Women and Infants
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1