Tur Rahardjo, W. Mailana, T. Kisnanto, Darlina, S. Nurhayati, D. Tetriana, D. Ramadhani, M. Syaifudin
{"title":"碱性单细胞凝胶电泳评价马木居(高本底辐射区)居民淋巴细胞DNA损伤","authors":"Tur Rahardjo, W. Mailana, T. Kisnanto, Darlina, S. Nurhayati, D. Tetriana, D. Ramadhani, M. Syaifudin","doi":"10.1504/IJLR.2017.10008590","DOIUrl":null,"url":null,"abstract":"Mamuju in Indonesia has a highest average dose rate and considered as a high background radiation area (HBRA) in Indonesia. Here in this research a preliminary study to evaluate DNA damage using the alkaline comet assay in inhabitants of Takandeang village, Mamuju, was conducted. Blood samples from 55 healthy donors living in Takandeang village and 22 control samples were obtained and processed into the assay. Results showed that there were insignificant differences of comet tail length (TL) and tail moment (TM) between Takandeang village inhabitants and control samples (p = 0.578, 0.174). Regression analysis revealed that the DNA damage was increased with age in control samples, even though the correlation was not significant (p > 0.05). In contrast a significant negative correlation (p = 0.02) was observed in Takandeang inhabitants. Results found in this research should be validating in a larger study using more samples from Mamuju.","PeriodicalId":14141,"journal":{"name":"International Journal of Low Radiation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Assessment of DNA damage in lymphocytes of Mamuju (a high background radiation area) inhabitants using alkaline single cell gel electrophoresis\",\"authors\":\"Tur Rahardjo, W. Mailana, T. Kisnanto, Darlina, S. Nurhayati, D. Tetriana, D. Ramadhani, M. Syaifudin\",\"doi\":\"10.1504/IJLR.2017.10008590\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Mamuju in Indonesia has a highest average dose rate and considered as a high background radiation area (HBRA) in Indonesia. Here in this research a preliminary study to evaluate DNA damage using the alkaline comet assay in inhabitants of Takandeang village, Mamuju, was conducted. Blood samples from 55 healthy donors living in Takandeang village and 22 control samples were obtained and processed into the assay. Results showed that there were insignificant differences of comet tail length (TL) and tail moment (TM) between Takandeang village inhabitants and control samples (p = 0.578, 0.174). Regression analysis revealed that the DNA damage was increased with age in control samples, even though the correlation was not significant (p > 0.05). In contrast a significant negative correlation (p = 0.02) was observed in Takandeang inhabitants. Results found in this research should be validating in a larger study using more samples from Mamuju.\",\"PeriodicalId\":14141,\"journal\":{\"name\":\"International Journal of Low Radiation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-10-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Low Radiation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1504/IJLR.2017.10008590\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Low Radiation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1504/IJLR.2017.10008590","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
Assessment of DNA damage in lymphocytes of Mamuju (a high background radiation area) inhabitants using alkaline single cell gel electrophoresis
Mamuju in Indonesia has a highest average dose rate and considered as a high background radiation area (HBRA) in Indonesia. Here in this research a preliminary study to evaluate DNA damage using the alkaline comet assay in inhabitants of Takandeang village, Mamuju, was conducted. Blood samples from 55 healthy donors living in Takandeang village and 22 control samples were obtained and processed into the assay. Results showed that there were insignificant differences of comet tail length (TL) and tail moment (TM) between Takandeang village inhabitants and control samples (p = 0.578, 0.174). Regression analysis revealed that the DNA damage was increased with age in control samples, even though the correlation was not significant (p > 0.05). In contrast a significant negative correlation (p = 0.02) was observed in Takandeang inhabitants. Results found in this research should be validating in a larger study using more samples from Mamuju.
期刊介绍：
The IJLR is a peer-reviewed journal dedicated to the publication of research articles, review papers and technical notes in all domains related to low-dose radiation, among which are the biological and health effects in humans and the biota, in vitro and in vivo research on low radiation effects, regulatory and policy aspects, risk estimation and public perception.
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