牛粪灰对豇豆种子防斑蚜潜力的研究(鞘翅目:金蝇科)

M. Suleiman, F. Haruna
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摘要

在室内温度为50-65%、温度为28-32℃的条件下,对牛粪灰(CDA)对豇豆种子的保护作用进行了研究。在培养皿中,将0.625%、1.25、2.50、5.00、10.00%(w/w)的CDA与0.625%的氯氰菊酯粉末以不同的比率掺入到20g豇豆种子中。没有将灰烬或氯氰菊酯添加到对照中。将十只甲虫放入每个培养皿中,并用平纹棉布覆盖。实验采用完全随机设计(CRD),重复三次。测定了治疗对成年死亡率的影响,以及CDA对斑蝥的半数致死剂量(LD50)。评估了不同剂量CDA处理的种子的成虫羽化和重量损失。研究结果显示,不同CDA治疗之间的黄斑C.maculatus成年死亡率差异显著(p<0.05),治疗后72小时(HAT)的死亡率在13.33%至100.00%之间。研究结果还表明,CDA在治疗后24小时(HAT)对黄斑C.maculatus的LD50为5.51%(w/w)。经处理的种子中未出现成虫,在用最低剂量处理的种子和仅用对照的种子中观察到体重减轻。因此,CDA可用于保护豇豆种子在贮藏过程中免受斑点线虫的侵扰。
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Potentials of Cow Dung Ash as Protectants of Cowpea Seeds against Callosobruchus maculatus F. (Coleoptera: Chrysomelidae)
Experiments were conducted to assess the protectant ability of cow dung ash (CDA) to cowpea seeds against Callosobruchus maculatus F. under laboratory conditions of 50-65% R.H and 28-32oC. The CDA at different rates of 0.625, 1.25, 2.50, 5.00, 10.00% (w/w) incorporated with cypermethrin powder at 0.625% were applied to 20 g of cowpea seeds in petri dishes. None of the ashes or cypermethrin was added to the control. Ten beetles were introduced into each of the petri dishes and covered with muslin cloth. The experiment was arranged in a completely randomized design (CRD) and replicated three times. Effects of the treatments on adult mortalities as well as median lethal dose (LD50) of CDA against C. maculatus were determined. Adult emergence and weight losses in seeds treated with CDA at different doses were assessed. Findings of the study revealed that the adult mortality of C. maculatus among different CDA treatments differed significantly (p < 0.05) and ranged from 13.33 to 100.00% 72 hours after treatment (HAT). Results of the study also showed that LD50 of CDA against C. maculatus at 24 hours after treatment (HAT) was 5.51 % (w/w). No adults emerged from the treated seeds and weight losses were observed in those treated with the lowest dose and the control only. Therefore, CDA could be utilized to protect cowpea seeds from C. maculatus infestations during storage.
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