{"title":"利用微卫星标记评价非洲万寿菊（Tagetes erecta）杂交种的遗传纯度","authors":"Chayanika Lahkar, Sushil Kumar Singh, Akhil Ranjan Baruah, Reena P. Borkakati","doi":"10.1007/s40003-023-00669-4","DOIUrl":null,"url":null,"abstract":"<div><p>The availability of information regarding breeding and molecular aspects of African marigold (<i>Tagetes erecta</i> L.) is rare, and a very few hybrids have been developed for this economically viable crop. A study was conducted using 13 parental genotypes (3 male sterile lines and 10 testers) of African marigold to develop 30 F<sub>1</sub> hybrids, and the true hybrids we evaluated based on phenotypic and marker-based genotypic data. Analysis of variance revealed significant diversity among the parental lines for different growth and flower traits. Fourteen SSR markers were used to test the purity of selected F<sub>1s</sub>, out of which two markers (TE57, T93B) showed polymorphism in the parental lines and cross combinations, with 2–5 alleles. The markers, TE57 and T93B revealed polymorphism information content (PIC) of 0.345 and 0.543 (mean 0.444), respectively, and detected a heterozygosity mean value of 0.528 in all, indicating the efficacy of microsatellite markers in discriminating the hybrids. The phenotypic data revealed significant difference in means for both quantitative and qualitative traits between the parents and the hybrids, in support of the molecular outcomes pertaining that hybrids detected through microsatellite markers were pure F<sub>1s</sub>. The study identified a set of compatible cross combinations that can be used to develop highly heterotic hybrid combinations with different growth and flowering characteristics.</p></div>","PeriodicalId":7553,"journal":{"name":"Agricultural Research","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Assessment of Genetic Purity in African Marigold (Tagetes erecta) Hybrids Using Microsatellite Markers\",\"authors\":\"Chayanika Lahkar, Sushil Kumar Singh, Akhil Ranjan Baruah, Reena P. Borkakati\",\"doi\":\"10.1007/s40003-023-00669-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The availability of information regarding breeding and molecular aspects of African marigold (<i>Tagetes erecta</i> L.) is rare, and a very few hybrids have been developed for this economically viable crop. A study was conducted using 13 parental genotypes (3 male sterile lines and 10 testers) of African marigold to develop 30 F<sub>1</sub> hybrids, and the true hybrids we evaluated based on phenotypic and marker-based genotypic data. Analysis of variance revealed significant diversity among the parental lines for different growth and flower traits. Fourteen SSR markers were used to test the purity of selected F<sub>1s</sub>, out of which two markers (TE57, T93B) showed polymorphism in the parental lines and cross combinations, with 2–5 alleles. The markers, TE57 and T93B revealed polymorphism information content (PIC) of 0.345 and 0.543 (mean 0.444), respectively, and detected a heterozygosity mean value of 0.528 in all, indicating the efficacy of microsatellite markers in discriminating the hybrids. The phenotypic data revealed significant difference in means for both quantitative and qualitative traits between the parents and the hybrids, in support of the molecular outcomes pertaining that hybrids detected through microsatellite markers were pure F<sub>1s</sub>. The study identified a set of compatible cross combinations that can be used to develop highly heterotic hybrid combinations with different growth and flowering characteristics.</p></div>\",\"PeriodicalId\":7553,\"journal\":{\"name\":\"Agricultural Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2023-08-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s40003-023-00669-4\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Research","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s40003-023-00669-4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
摘要
有关非洲万寿菊（Tagetes erecta L.）育种和分子方面的信息很少，为这种经济上可行的作物培育的杂交种也很少。一项研究利用非洲万寿菊的 13 个亲本基因型（3 个雄性不育系和 10 个测试者）培育了 30 个 F1 代杂交种，并根据表型和基于标记的基因型数据对真正的杂交种进行了评估。方差分析显示，亲本品系在不同的生长和花卉性状上存在显著差异。我们使用了 14 个 SSR 标记来检测所选 F1 的纯度，其中两个标记（TE57 和 T93B）在亲本品系和杂交组合中表现出多态性，有 2-5 个等位基因。TE57 和 T93B 标记的多态性信息含量（PIC）分别为 0.345 和 0.543（平均值为 0.444），检测到的杂合度平均值为 0.528，表明微卫星标记在区分杂交种方面的功效。表型数据显示，亲本和杂交种的数量和质量性状均有显著差异，支持通过微卫星标记检测到的杂交种为纯合 F1 的分子结果。这项研究确定了一组相容的杂交组合，可用于培育具有不同生长和开花特性的高异交杂交组合。
Assessment of Genetic Purity in African Marigold (Tagetes erecta) Hybrids Using Microsatellite Markers
The availability of information regarding breeding and molecular aspects of African marigold (Tagetes erecta L.) is rare, and a very few hybrids have been developed for this economically viable crop. A study was conducted using 13 parental genotypes (3 male sterile lines and 10 testers) of African marigold to develop 30 F1 hybrids, and the true hybrids we evaluated based on phenotypic and marker-based genotypic data. Analysis of variance revealed significant diversity among the parental lines for different growth and flower traits. Fourteen SSR markers were used to test the purity of selected F1s, out of which two markers (TE57, T93B) showed polymorphism in the parental lines and cross combinations, with 2–5 alleles. The markers, TE57 and T93B revealed polymorphism information content (PIC) of 0.345 and 0.543 (mean 0.444), respectively, and detected a heterozygosity mean value of 0.528 in all, indicating the efficacy of microsatellite markers in discriminating the hybrids. The phenotypic data revealed significant difference in means for both quantitative and qualitative traits between the parents and the hybrids, in support of the molecular outcomes pertaining that hybrids detected through microsatellite markers were pure F1s. The study identified a set of compatible cross combinations that can be used to develop highly heterotic hybrid combinations with different growth and flowering characteristics.
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The main objective of this initiative is to promote agricultural research and development. The journal will publish high quality original research papers and critical reviews on emerging fields and concepts for providing future directions. The publications will include both applied and basic research covering the following disciplines of agricultural sciences: Genetic resources, genetics and breeding, biotechnology, physiology, biochemistry, management of biotic and abiotic stresses, and nutrition of field crops, horticultural crops, livestock and fishes; agricultural meteorology, environmental sciences, forestry and agro forestry, agronomy, soils and soil management, microbiology, water management, agricultural engineering and technology, agricultural policy, agricultural economics, food nutrition, agricultural statistics, and extension research; impact of climate change and the emerging technologies on agriculture, and the role of agricultural research and innovation for development.
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